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人可溶性晚期糖基化终末产物受体（sRAGE）ELISA检测试剂盒
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详细信息
人可溶性晚期糖基化终末产物受体（sRAGE）ELISA检测试剂盒
●英文名称：Human sRAGE ELISA Kit
●产品规格：96T（人份）/48T（人份）
●单孔检测：96T，可以检测90份样本，留5个孔做标准曲线，一个孔做空白对照；
48T，可以检测42份样本，留5个孔做标准曲线，一个孔做空白对照；
●品牌：子科生物ZIKER,美国R&D,美国immonoway,美国sciencell,德国IBL
●适用范围：实验结果仅供科研参考，不推荐用于临床诊断结果。
人可溶性晚期糖基化终末产物受体（sRAGE）ELISA检测试剂盒操作步骤:
1. 从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。
2. 设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；
3. 样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。
4. 除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。
5. 弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。
6. 每孔加入底物A、B各50μL，37℃避光孵育15min。
7. 每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。
试剂盒性能:
1. 准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。
2. 灵敏度：zui低检测浓度小于1.0 pg/mL。
3. 特异性：不与其它可溶性结构类似物交叉反应。
4. 重复性：板内、板间变异系数均小于15%。
5. 贮藏：2-8℃，避光防潮保存。
6. 有效期：6个月
结果判断:
绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。
6000-5005 Micro Well Strips
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9243547 Pyro Q24, PM Service "
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Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
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9242770 Belt, x-drive, portal, AS, Qsym
9242698 RGQ, Full Agreement "Repair service delivery: send to Regional Repair Center
Service response time (repair): 7-10 days turnaround time
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for repair parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) provision: yes, included (provision time 2 days)
Transportation cost coverage: yes, included"
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9240744 Belt, Reagent Pump , Long, AP96
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9240698 Cap, Waste Bottle, Modified, AP
9243655 Addl Unit or Protocol, Comp. Valid Supp Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Comprehensive validation service catalog no(s). Scope of work defined from the primary unit utilized in the Comprehensive Validation Support. Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.
1024481 #LCX Vacuum System Holds 24 QIAamp Maxi columns includes connectors, tubing, waste bottles, vacuum gauge, filters, tray, user manual.
9241221 RCS, Installation RCS Installation facilitates the quick start up of the RCS into the lab routine. It covers hardware and software installation of the system and provides the user with an introductory training on operation and user maintenance. Installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
330025 Microbial DNA qPCR Assays
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