Appearance:

yellowish crystals

Molecular weight:

458.42

Molecular formula:

C₂₄H₁₈N₄O₆

Solubility:

soluble in polar organic solvents (DMF, DMSO, alcohols)

Quality control:

NMR ¹H, HPLC-MS (95%)

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Excitation maximum, nm:

494

ε, L⋅mol⁻¹⋅cm⁻¹:

75000

Emission maximum, nm:

520

Fluorescence quantum yield:

0.9

CF₂₆₀:

0.20

CF₂₈₀:

0.17

荧光染料示例

激发光

Indo-1, Ca saturated

331 nm

Indo-1 Ca2+

346 nm

Cascade Blue BSA pH 7.0

401 nm

Cascade Blue

398 nm

LysoTracker Blue

373 nm

Alexa 405

401 nm

LysoSensor Blue pH 5.0

374 nm

LysoSensor Blue

374 nm

DyLight 405

399 nm

DyLight 350

332 nm

BFP (Blue Fluorescent Protein)

380 nm

Alexa 350

343 nm

7-Amino-4-methylcoumarin pH 7.0

346 nm

Amino Coumarin

345 nm

AMCA conjugate

347 nm

Coumarin

360 nm

7-Hydroxy-4-methylcoumarin

360 nm

7-Hydroxy-4-methylcoumarin pH 9.0

361 nm

6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0

358 nm

Hoechst 33342

352 nm

Pacific Blue

404 nm

Hoechst 33258

352 nm

Hoechst 33258-DNA

352 nm

Pacific Blue antibody conjugate pH 8.0

404 nm

PO-PRO-1

434 nm

PO-PRO-1-DNA

435 nm

POPO-1

433 nm

POPO-1-DNA

433 nm

DAPI-DNA

359 nm

DAPI

358 nm

Marina Blue

362 nm

SYTOX Blue-DNA

445 nm

CFP (Cyan Fluorescent Protein)

434 nm

eCFP (Enhanced Cyan Fluorescent Protein)

437 nm

1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS)

375 nm

Indo-1, Ca free

346 nm

1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid)

375 nm

BO-PRO-1-DNA

462 nm

BOPRO-1

462 nm

BOBO-1-DNA

461 nm

SYTO 45-DNA

451 nm

evoglow-Pp1

448 nm

evoglow-Bs1

448 nm

evoglow-Bs2

448 nm

Auramine O

431 nm

DiO

487 nm

LysoSensor Green pH 5.0

447 nm

Cy 2

489 nm

LysoSensor Green

447 nm

Fura-2, high Ca

336 nm

Fura-2 Ca2+sup>

336 nm

SYTO 13-DNA

488 nm

YO-PRO-1-DNA

491 nm

YOYO-1-DNA

491 nm

eGFP (Enhanced Green Fluorescent Protein)

488 nm

LysoTracker Green

503 nm

GFP (S65T)

489 nm

BODIPY FL, MeOH

502 nm

Sapphire

396 nm

BODIPY FL conjugate

503 nm

MitoTracker Green

490 nm

MitoTracker Green FM, MeOH

490 nm

Fluorescein 0.1 M NaOH

493 nm

Calcein pH 9.0

494 nm

Fluorescein pH 9.0

490 nm

Calcein

493 nm

Fura-2, no Ca

367 nm

Fluo-4

494 nm

FDA

495 nm

DTAF

495 nm

Fluorescein

495 nm

Fluorescein antibody conjugate pH 8.0

493 nm

CFDA

495 nm

FITC

495 nm

Alexa Fluor 488 hydrazide-water

493 nm

DyLight 488

493 nm

5-FAM pH 9.0

492 nm

FITC antibody conjugate pH 8.0

495 nm

Alexa 488

493 nm

Rhodamine 110

497 nm

Rhodamine 110 pH 7.0

497 nm

Acridine Orange

431 nm

Alexa Fluor 488 antibody conjugate pH 8.0

499 nm

BCECF pH 5.5

485 nm

PicoGreendsDNA quantitation reagent

502 nm

SYBR Green I

498 nm

Rhodaminen Green pH 7.0

497 nm

CyQUANT GR-DNA

502 nm

NeuroTrace 500/525, green fluorescent Nissl stain-RNA

497 nm

DansylCadaverine

335 nm

Rhodol Green antibody conjugate pH 8.0

499 nm

Fluoro-Emerald

495 nm

Nissl

497 nm

Fluorescein dextran pH 8.0

501 nm

Rhodamine Green

497 nm

5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0

504 nm

DansylCadaverine, MeOH

335 nm

eYFP (Enhanced Yellow Fluorescent Protein)

514 nm

Oregon Green 488

498 nm

Oregon Green 488 antibody conjugate pH 8.0

498 nm

Fluo-3

506 nm

BCECF pH 9.0

501 nm

SBFI-Na+

336 nm

Fluo-3 Ca2+

506 nm

Rhodamine 123, MeOH

507 nm

FlAsH

509 nm

Calcium Green-1 Ca2+

506 nm

Magnesium Green

507 nm

DM-NERF pH 4.0

493 nm

Calcium Green

506 nm

Citrine

515 nm

LysoSensor Yellow pH 9.0

335 nm

TO-PRO-1-DNA

515 nm

Magnesium Green Mg2+

507 nm

Sodium Green Na+

507 nm

TOTO-1-DNA

514 nm

Oregon Green 514

512 nm

Oregon Green 514 antibody conjugate pH 8.0

513 nm

NBD-X

466 nm

DM-NERF pH 7.0

509 nm

NBD-X, MeOH

467 nm

CI-NERF pH 6.0

513 nm

Alexa 430

431 nm

Alexa Fluor 430 antibody conjugate pH 7.2

431 nm

CI-NERF pH 2.5

504 nm

Lucifer Yellow, CH

428 nm

LysoSensor Yellow pH 3.0

389 nm

6-TET, SE pH 9.0

521 nm

Eosin antibody conjugate pH 8.0

525 nm

Eosin

524 nm

6-Carboxyrhodamine 6G pH 7.0

526 nm

6-Carboxyrhodamine 6G, hydrochloride

525 nm

Bodipy R6G SE

528 nm

BODIPY R6G, MeOH

528 nm

6 JOE

520 nm

Cascade Yellow antibody conjugate pH 8.0

399 nm

Cascade Yellow

399 nm

mBanana

540 nm

Alexa Fluor 532 antibody conjugate pH 7.2

528 nm

Alexa 532

528 nm

Erythrosin-5-isothiocyanate pH 9.0

533 nm

6-HEX, SE pH 9.0

534 nm

mOrange

548 nm

mHoneydew

478 nm

Cy 3

549 nm

Rhodamine B

543 nm

DiI

551 nm

5-TAMRA-MeOH

543 nm

Alexa 555

553 nm

Alexa Fluor 555 antibody conjugate pH 7.2

553 nm

DyLight 549

555 nm

BODIPY TMR-X, SE

544 nm

BODIPY TMR-X, MeOH

544 nm

PO-PRO-3-DNA

539 nm

PO-PRO-3

539 nm

Rhodamine

551 nm

Bodipy TMR-X conjugate

544 nm

POPO-3

533 nm

Alexa 546

562 nm

BODIPY TMR-X antibody conjugate pH 7.2

544 nm

Calcium Orange Ca2+

549 nm

TRITC

550 nm

Calcium Orange

549 nm

Rhodaminephalloidin pH 7.0

558 nm

MitoTracker Orange

551 nm

MitoTracker Orange, MeOH

551 nm

Phycoerythrin

565 nm

Magnesium Orange

550 nm

R-Phycoerythrin pH 7.5

565 nm

5-TAMRA pH 7.0

553 nm

5-TAMRA

549 nm

Rhod-2

552 nm

FM 1-43

472 nm

Rhod-2 Ca2+

553 nm

Tetramethylrhodamine antibody conjugate pH 8.0

552 nm

FM 1-43 lipid

473 nm

LOLO-1-DNA

568 nm

dTomato

554 nm

DsRed

563 nm

Dapoxyl (2-aminoethyl) sulfonamide

372 nm

Tetramethylrhodamine dextran pH 7.0

555 nm

Fluor-Ruby

554 nm

Resorufin

571 nm

Resorufin pH 9.0

571 nm

mTangerine

568 nm

LysoTracker Red

578 nm

Lissaminerhodamine

572 nm

Cy 3.5

578 nm

Rhodamine Red-X antibody conjugate pH 8.0

573 nm

Sulforhodamine 101, EtOH

578 nm

JC-1 pH 8.2

593 nm

JC-1

592 nm

mStrawberry

575 nm

MitoTracker Red

578 nm

MitoTracker Red, MeOH

578 nm

X-Rhod-1 Ca2+

580 nm

Alexa Fluor 568 antibody conjugate pH 7.2

579 nm

Alexa 568

576 nm

5-ROX pH 7.0

578 nm

5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt)

578 nm

BO-PRO-3-DNA

574 nm

BOPRO-3

574 nm

BOBO-3-DNA

570 nm

Ethidium Bromide

524 nm

ReAsH

597 nm

Calcium Crimson

589 nm

Calcium Crimson Ca2+

590 nm

mRFP

585 nm

mCherry

587 nm

Texas Red-X antibody conjugate pH 7.2

596 nm

HcRed

590 nm

DyLight 594

592 nm

Ethidium homodimer-1-DNA

528 nm

Ethidiumhomodimer

528 nm

Propidium Iodide

538 nm

SYPRO Ruby

467 nm

Propidium Iodide-DNA

538 nm

Alexa 594

590 nm

BODIPY TR-X, SE

588 nm

BODIPY TR-X, MeOH

588 nm

BODIPY TR-X phallacidin pH 7.0

590 nm

Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2

567 nm

YO-PRO-3-DNA

613 nm

Di-8 ANEPPS

469 nm

Di-8-ANEPPS-lipid

469 nm

YOYO-3-DNA

612 nm

Nile Red-lipid

553 nm

Nile Red

559 nm

DyLight 633

624 nm

mPlum

587 nm

TO-PRO-3-DNA

642 nm

DDAO pH 9.0

648 nm

Fura Red, high Ca

434 nm

Allophycocyanin pH 7.5

651 nm

APC (allophycocyanin)

650 nm

Nile Blue, EtOH

631 nm

TOTO-3-DNA

642 nm

Cy 5

646 nm

BODIPY 650/665-X, MeOH

646 nm

Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2

569 nm

DyLight 649

652 nm

Alexa Fluor 647 antibody conjugate pH 7.2

653 nm

Alexa 647

653 nm

Fura Red Ca2+

435 nm

Atto 647

644 nm

Fura Red, low Ca

472 nm

Carboxynaphthofluorescein pH 10.0

600 nm

Alexa 660

664 nm

Alexa Fluor 660 antibody conjugate pH 7.2

663 nm

Cy 5.5

673 nm

Alexa Fluor 680 antibody conjugate pH 7.2

679 nm

Alexa 680

679 nm

DyLight 680

678 nm

Alexa Fluor 700 antibody conjugate pH 7.2

696 nm

Alexa 700

696 nm

FM 4-64, 2% CHAPS

506 nm

FM 4-64

508 nm