齐一生物大鼠脑源性神经营养因子（BDNF）检测试剂盒齐一生物科技（上海）有限公司以真诚、主动、周到、规范、热情的服务很快得到广大客户的*好评。公司秉承“适应多样化的需求，提供专业化的服务”的经营理念，为客户提供*的服务。欢迎社会各界朋友前来洽谈业务，共创辉煌

ELISA试剂盒操作步骤
ELISA试剂盒使用前,将所有试剂充分混匀.不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差.
根据待测样品数量加上标准品的数量决定所需的板条数.每个标准品和空白孔建议做复孔.每个样品根据自己的数量来定,能使用复孔的尽量做复孔.标本用标本稀释液1：1稀释后加入50ul于反应孔内.
加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内.立即加入50ul的标记的抗体.盖上膜板,轻轻振荡混匀,37℃温育1小时.
甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟.
齐一生物大鼠脑源性神经营养因子（BDNF）检测试剂盒甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入底物A、B各50ul,轻轻振荡混匀,37℃温育10分钟.避免光照.
取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果.
在450nm波长处测定各孔的OD值.
9022022    Sensor, Z-axis, with cable, QXA    
9021653    Kit, stickers, eluate drawer, Qsym    
9021623    Gateway 2, CC-Link, DCU    
9021289    Yellow grease for PM, EZ1    
9240671    Reservoir, 500ml w/cap, AP96/196    
9240640    Opto Sensor, Interlocks/Latch Lock, AP    
9240639    Opto Sensor, Common, AP    
9240633    Power Supply, International, AP97    
9240614    Fuse, 0.25A, FNM, AP96    
9240613    Fuse, 15.0A, FNM, AP96    
9240585    Filter Assembly, Pressure, AP196/98    
9240580    Cable, Vacuum Airflow Sensor, AP196/98    
9232772    Syringe, 2.5ml    
9232770    Syringe, 500 μl    
9239346    Spotlight bulb (2), BR M48    
9239334    Silicone Grease, BR M48 & BR EZ1    
9236466    BR 8000, Full Agreement    The Full Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9236435    Power entry replacement kit, TWI    
9239139    Spacer, support & pod foot, TWII    
9239104    Washer set,  valve Port S 5.3mm, Kloehn    
9239102    Washer set, valve port A/B 4.5mm, Kloehn    
9232649    Cooling block, 1-tube 13 ml    Holder for accommodating a single tube (13 ml) on the worktable
9232640    Box, used tips    
9232635    Bottle, 125-ml, orange fitting    
9232633    Bottle, 125-ml, white luer adapter    
9238990    Z-Axis Coupling Unit, EZ1    
9238924    Bearing, BS15    
9238923    Hinge support, BS15    
9238784    Swing-out rotor for 6 buckets    
9238909    PCB, UI-01 w. LCD/Keypad, BS96    
9238740    SBS Vacuum Manifold Top Station    
9238713    Eprom 4-15, Zent1, opt. Imbalance    
9232329    Cable, LSB to X-main board, BR8000    
9232321    Cable set, PS5 to XMAIN, BR8000    
9232276    Motor, Z, BR8000    
9238698    Eprom 4K15, Zent1, imbalance sensor    
9231596    Fitting, 3/2,    
9231131    Flat Band Cable 10P Xadp - Yzadp    
9231130    Cable, X-adapter to X-motor, power, BR80    
9014778    Pump, vacuum, auto vac, UK,  BR8000    
9020078    Keypad, TLyser 2    
9020075    V-Belt, Motor, TLyser 2    
9020072    Foot Cover (1), TLyser 2    
9020068    Hall Sensor Board, TLyser    
9015015    Probe Guide, BR8 RD v1.0    
9014852    Rotor unit, BR8000    
9014784    Laboratory Cabinet MDx    
9014713    Cable, Rotor Door Sensor, BR8 RD    
9022198    PyroMark Q96 ID App Software 2.5    
9021403    RGQ MDx User manuals for IVD use  (US)    
9019885    Cover, main, label Biocontrol, RG3    
9019745    EZ1 Adv. XL DNA Dried Blood Card    
9019729    Board, motor control, Z, V2, QX    
9013842    Tip Adapter Kit, 1100ul, BR8000    
9013550    Set, Fuses,  (220 + 110V) Plato 1/3    
9013429    Maintenance kit, semi annual, BR9600 (C)    
9013425    Maintenance Kit BR 9604    "See Components tab for what comes in this kit.
Also includes 4x9013641, BGR Valve 4-port for P1/3 Syringe Pump; 1.5m of 9233214 (listed in Components as qty of 1)."
9019668    PCB - Valve, PyroQ24    
9019649    Fuse kit (6.3Ax10, 5Ax5), PyroQ96    
9019602    Home flag, Y-axis, Cx8    
9019599    Housing, filter seal, CX1    
9019585    Slideout electrical tray, QGY    
9015283    Cover, Z, M48
9015274    Laboratory Cabinet BR8000RD
9001909    QIAgility HEPA/UV (w/o PC) Priority    QIAgility HEPA/UV (w/o PC) Priority includes the QIAgility instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor and travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
133118    NeXtalStock tert-Butanol (200)
133109    NeXtalStock Sodium chloride (200)
965652    QIAamp Virus BioRobot MDx Kit (12)    For 12 x 96 preps: 12 QIAamp 96 Plates,RNase-free Buffers,† QIAGEN Protease,Elution Microtubes CL, Caps, S-Blocks, Carrier RNA
965162    QIAamp DNA Blood BioRobot 9604 Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
962341    DirectPrep 96 BioRobot Kit (4)    For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Reagents, Buffers, Flat-Bottom Blocks and Lids, 96-Well Microplates FB, AirPore Tape Sheets, Tape Pads
9018535    Tool, door sensor deactivation, QIAC
133103    NeXtalStock Propylene glycol (200)
9015513    PM Kit Biorobot M 48
9015470    Motor, Z
9001887    QIAcube (230V), PrioPLUS    "QIAcube (230V) Instrument 
Installation of QIAcube  instrument & Introductory training 
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001886    QIAcube (230V), Priority    QIAcube (230V), Priority includes the QIAcube instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor and travel costs with a service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
9018459    Safety valve, assy, QX
9018456    Linear guide, Y axis, QX
9012421    MP-Slot Extention
9012397    Holder, vacuum trap, auto vac, BR8000
204854    QuantiFast Multiplex RT-PCR Kit (400)
133174    NeXtalStock CHES pH 10.0 (200)
133173    NeXtalStock CHES pH 9.0 (200)
291043    mericon Screen nos Kit (24)
290135    mericon Vibrio triple Kit (96)
290115    mericon Y. enterocolitica Kit (96)
9015402    O-rings, plunger, M96 (8)
9001731    QIAgility HEPA / UV Instrument
133088    NeXtalStock PEG 550 MME (200)
38025    MAL Cartridge (5ml)
292015    mericon Pig Kit (96)
133123    NeXtalStock Tris (200)
965672    QIAamp One-For-All Nucleic Acid Kit (12)
133192    NeXtalStock Ammonium tartrate pH 7 (200)
133183    NeXtalStock Citric acid pH 3.5 (200)
9018513    Adapter, USB-serial, EZ1a
9001896    QIAxtractor, UV/HEPA (w/o PC) System    QIAxtractor, UV/HEPA (w/o PC) System combines the QIAxtractor system and installation. The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation and user maintenance. All travel and labor cost included for Installation. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
51104    QIAamp DNA Blood Mini Kit (50)    For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
133099    NeXtalStock Potassium nitrate (200)
133092    NeXtalStock PEG 8,000 (200)
9015516    Heat unit, complete
9018452    Piston, purge (1), QX
9012416    Microplate Station 8000, short    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the BioRobot 8000 or MDx worktable
133168    NeXtalStock CAPSO pH 9.2 (200)
9018424    Door, N2, QX
9015405    Board, PDB, M96