起订量:
齐一生物大鼠白细胞介素1β(IL-1β)检测试剂盒
初级会员第11年
生产厂家齐一生物科技(上海)有限公司,专业从事生物产品研发、生产、经营为一体的专业化生物工程技术公司!为广大科研工作者、企业和学校提供质优价廉的实验室用品,本公司主要经营ELISA酶联免疫检测试剂盒、进口原装ELISA试剂盒、分子生物学检测试剂盒、免疫学检测试剂盒、各品牌*抗体抗原、细胞菌株、生物培养基、生化试剂、实验耗材等多门类上万余种科研试剂产品!产品涉及分子生物学、细胞生物学、免疫学等生命科学的各个领域!已迅速发展成为国内科学试剂的运营商之一,是国家重点实验室*供应商,并与国内多家科研单位紧密合作。
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齐一生物大鼠白细胞介素1β(IL-1β)检测试剂盒齐一生物科技(上海)有限公司以真诚、主动、周到、规范、热情的服务很快得到广大客户的*好评。公司秉承“适应多样化的需求,提供专业化的服务”的经营理念,为客户提供*的服务。欢迎社会各界朋友前来洽谈业务,共创辉煌
齐一生物大鼠白细胞介素1β(IL-1β)检测试剂盒标本的采集及保存:
1.血清:室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存 过程 中 如有沉淀形成,应再次离心。
2.血浆:应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
3.尿液:用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。
4.细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/ 分)。仔细收集上清。 检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以 使细胞破坏并 放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5.组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
标本要求:
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
9019540 Kit, screws & washers, inst. covers, Cxx
9019512 Tube, pinch valve, CX1
9019503 TANK LOWER BUFFER ASSY GS
217061 miRNeasy 96 Kit (4)
204257 QuantiFast Probe PCR Kit (4000)
9019391 Fan, cooler blower, RG3
9019384 Board, main, dual & OEM, RG3
9019356 Sensor, rotor home, RG6
9019338 LED, red (625), RG6
9019332 PCB, Power w/regulator trans, RG6
9019312 Solenoid, door lock, EZ1xl
9019235 Linear guide - Y, PyroQ24
9234680 Rack for Reagents, Plastic block 40 h
9020476 Fan, GSC
9233430 Belt, tooth, Z-drive, BR8000
9019198 Damper kit - Top cover, PyroQ96MD
9019190 Bellows, PyroQ96MD
9019189 Cable - Camera, PyroQ96
1020952 Protease Resuspension Buffer (6/15),KG
1019266 QIAquick 96 PB Buffer Module 8000
1054575 HiSpeed Plasmid Giga EF Kit (5)
1061072 AIV-H5 RT-PCR REAGENT
1061094 HCV RT-PCR REAGENT VII
1061115 PG FMDV RT-PCR Assay (48) Z
9016711 Security Key, LC Analyzer, encoded
9016685 Washer Mechanical, RCS1
9016644 RoboVac ground plate&contact pins, BR8 I
9016539 Transportation box, BR M48 Accessories
9016527 Transportation box, Lab. Cabinet
9016484 Adapter PCB, Standart, Kloehn
9019889 Transport box, cardboard, EZ1a
9019864 Waste sink mat support, QXT
9019862 Low skirt transfer carriage, CX1
9019761 Power switch, 6A, 250VAC, V2, QX
9001532 QIAgility System HEPA / UV (incl.Laptop)
9013455 Kit, service, BioRobot 9600
9013430 Maintenance kit, semi annual, BR9600 (D)
9238809 Round carrier for 4 Falcon tubes 50ml
9238806 Round carrier for 9 Falcon tubes 15ml
9019664 PyroMark Vac Prep Worktable, PyroQ96VW
9019652 O rings - VPT Filter Probe, PyroQ96VW
9019639 Magnet, door lock, bottom, EZ1xl
9019613 Home flag, X-axis, Cxx
9019584 Gasket, fan, Cxx
9019570 Clamp, cable 3 wires 2 tabs thru, Cxx
9019567 Clamp Bracket, Y-arm mounting, Cxx
9019549 Pin, lid hinge, CAS
9019522 Plate Grippers RD, RCS
218161 miScript II RT Kit (50)
218075 miScript SYBR Green PCR Kit (1000)
9019430 Hinge, front rear hinge plates, RG3
9019398 Solenoid, lid lock, RG3
9019395 Fan, 120mm, rear panel, RG3
9019383 Board, main, standard, RG3
9019366 Wiring, lower heater, RG6
9019365 Solenoid, lid lock assembly, RG6
9019349 Sensor, heater fan speed, RG6
1027292 AllStars Neg. siRNA AF 488 (20 nmol)
9019240 Switch - ON/OFF, PyroQ24
9234698 Manual WinRufas 2.5 (CD)
9012416 Microplate Station 8000, short Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the BioRobot 8000 or MDx worktable
133168 NeXtalStock CAPSO pH 9.2 (200)
9018424 Door, N2, QX
9015405 Board, PDB, M96
9015386 Manual, Service, BIOROBOT MDx
9001863 Rotor-Gene Q 2plex HRM (PC) Priority Rotor-Gene Q 2plex HRM (PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents.
9001720 Rotor-Gene Q 6Plex no PC
9012331 Set, Tubing, Pipetting, Plato3xx1
133084 NeXtalStock PEG 300 (200)
38065 #PNA Cartridge (5ml)
204752 QuantiFast Multiplex PCR +R Kit (80)
290095 mericon Quant. L. pneumophila Kit (96)
800423 Library, Large Insert, Picked Clones
290045 mericon Campylobacter triple Kit (96)
9015353 Controller, temp.
133051 NeXtalStock HEPES sod. salt untit. (200)
9018388 QIAcube Tip Adapter Ring Tool
9018377 Teaching Tools, Alignment, AP
9012226 Power Supply, 6 amp for Plato size 90
9012066 Tray, buffer bottles, roter, BR8000
9001674 PyroMark Q96 MD Automated Instrument For performing automated pyrosequncing reaction on the Q96 MD
9001658 Rotor-Gene Q 5plex HRM (w/o PC) Priority Rotor-Gene Q 5plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents.
951054 EZ1 DNA Blood 350 μl Kit (48) "
48 Reagent Cartridges (Blood 350 ul), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml)"
9001874 EZ1 Advanced XL, System EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument and installation. The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation and user maintenance. All travel and labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
9015466 Cover, P
9012413 Z-Bars Gripper, RCS1
9012410 Tischplatte Plato 3301S Technikk (9194)
205110 Omniscript RT Kit (10) For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water
204845 QuantiTect Multiplex RT-PCR NR Kit(1000)
133178 NeXtalStock Citric acid pH 2.6 (200)
133165 NeXtalStock CAPS pH 11.4 (200)
9018409 Regulator, N2, internal, front, QX
9015406 Driver, motor, M96
9001861 Rotor-Gene Q 2plex (PC) Priority Rotor-Gene Q 2plex (PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents.
9012327 Peristaltic Pump Outlet Tubing
1067925 Buffer AW1 (175 ml)
38045 GNA Cartridge (5ml)
38003 ConA Cartridges (3 x 1ml)
9015365 Drop catcher, M96
204741 QuantiTect Multiplex PCR NoROX Kit (40) "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water
For quantitative, real-time PCR and two-step RT-PCR using probe detection with multiple assays."
133066 NeXtalStock Manganese(II) chloride (200)
290065 mericon Cronobacter spp Kit (96)
204374 Rotor-Gene Probe PCR Kit (400) The Rotor-Gene Probe PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time PCR and two-step RT-PCR using sequence-specific probes.
9015354 Springs, gas (2)
133054 NeXtalStock Imidazole HCl (200)
9001671 PyroMark Q24 Instrument For methylation and mutation analysis using Pyrosequencing technology in 24 well
950901 Capture Plate, standard yield (5)
37430 PhosphoSerine Antibody Q5 100 ug mixture of anti-phosphoserine antibodies(isotypes mouse IgG1 and IgM, for 200 ml working solution)
133030 NeXtalStock Cesium sulfate (200)
9001627 Rotor-Gene Q 2plex (w/o PC) System Rotor-Gene Q 2plex (w/o PC) System Priority includes the Rotor-Gene system and installation. The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware and trains the user on operation of the Rotorgene software. All travel and labor cost included for Installation and service documented on a GMP/GLP-compliant Field Service document.
9001620 Rotor-Gene Q 2plex System
9001603 Rotor-Disc 100 Starter Kit (230V)
9015316 Belt, Y-motor, v2, M48
9015312 Unit, spotlight, right, M48
9015309 Block, heater, M48
133140 NeXtalStock 1,2 Propanediol (200)
133020 NeXtalStock BIS-TRIS Propane (200)
1043368 QIAamp DNA Blood Mini Accessory Set A
133161 NeXtalStock CAPS pH 10.6 (200)
31326 Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgMu, 1 mL 1 ML
31327 Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (Fc) 1.0 ML
31328 Pierce(R) Phosphtase Conjugated Goat Anti-Mouse IgG + IgM 1 ML
31329 Pierce(R) Phosphatase Conjuga ted Rabbit Anti-Mouse IgG (H + 1.0 ML
31330 Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG+IgM (H 1 ML
31331 Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG {F(a 1.0 ML
31332 Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (Fc) 1.0 ML
31333 Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgM (u) 1.0 ML
31334 Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (H+L 0.5 ML
31335 Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG + Ig 1.0 ML
31337 Pierce(R) Phosphatase Conjugated Rabbit Anti-Goat IgG (Fc) 1.0 ML
31340 Pierce(R) Goat Anti-Rabbit IgG, (H+L), Alkaline Phosphatase 1 ML
31341 ImmunoPure Phosphatase Conjugated Goat Anti-Rabbit IgG (Fc) 1.0 ML
31342 Pierce(R) Phosphatase Conjuga ted Goat ANti-Rabbit IgG (H+L) 1 ML
31343 Pierce(R) Phosphatase Conjugated Goat Anti-Rabbit IgG 1.0 ML
31345 ImmunoPure Phosphatase Conj. Donkey Anti-Rabbit IgG(H+L)(min 0.5 ML
31346 Pierce Goat Anti-Rabbit IgG (H+L) Alkaline Phosphatase 0.2 MG
31350 Pierce(R) Phosphatase Conjugated Goat Anti-RatIgG (H+L), 1 1 ML
31360 Pierce(R) Phosphatase Conjugated Rabbit Anti-Sheep IgG (H+L) 1 ML
31391 Alkaline Phosphatase 20 mg
31392 Alkaline Phosphatase 100 mg
31400 Pierce(R) Peroxidase Conjugated Mouse Anti-Goat IgG (H+L) 1 ML
31401 Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Chicken IgY (H+ 1.5 ML
31402 Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG (H+L), 1.5 ML
31403 Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG {F(ab' 1.5 ML
31410 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L), 2 ML
31412 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L)(m 1.5 ML
31413 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG FC 1 ML
31414 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG F(ab')2 1.5 ML
31415 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgM (Fc5u) 2 ML
31417 Pierce(R) Peroxidase Conjugated Goat Anti-Human IgAa, 2 ML 2 ML
31418 Pierce(R) Peroxidase Conjugated Goat Anti-Human Immunoglobul 2 ML
31420 Pierce(R) Peroxidase Conjugated Mouse Anti-Human IgG)H+L)(m 1.5 ML
31423 Pierce(R) Peroxidase Conjugated Rabbit Anti-Human IgG (Fc) 1.5 ML
31430 Pierce(R) Goat Anti-Mouse IgG,(H&L), Peroxidase Conjugated, 2 ML
31431 ImmunoPure Peroxidase Conjugated Goat Anti-Mouse 0.2 ML
31432 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (H+L) 1.5 ML
31433 Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Goat IgG (Fc) 1.5 ML
31436 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG F(ab')2 2 ML
31437 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc) 2.0 ML
31438 Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anit- 0.5 ML
31439 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc) (mi 1.5 ML
31440 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgMu, 2 ML 2 ML
31444 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+ 2 ML
31446 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+L 1.5 ML
31448 Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anti- 0.5 ML
31450 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (H+L) 1.5 ML
31451 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG {F(ab 1.5 ML
31452 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (H+L) 1 ML
31453 Pierce(R) Mouse Transferrin Peroxidase Conjugated, 1 mg 1 MG
31455 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 ML