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人肠碱性磷酸酶（ALPI）ELISA检测试剂盒
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详细信息
人肠碱性磷酸酶(ALPI)ELISA检测试剂盒子科生物现货供应，子科生物专业生产研发经营稳定、优质、高效、实用的ELISA试剂盒，本公司可以提供ELISA试剂盒免费代测，ELISA试剂盒批发价零售，是ELISA试剂盒专业生产厂家，并代理各大进口原装品牌ELISA试剂盒，货期短，*，原装正品，！
报价，检测原理，使用说明书，咨询免费索要！
人肠碱性磷酸酶(ALPI)ELISA检测试剂盒操作步骤：
1. 使用前，将所有试剂充分混匀。不要使液体产生大量的泡沫，以免加样时加入大量的气泡，产生加样上的误差。
2. 根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定，能使用复孔的尽量做复孔。
3. 加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内。立即加入50ul的生物素标记的抗体。盖上膜板，轻轻振荡混匀，37℃温育45分钟。
4. 甩去孔内液体，每孔加满洗涤液，振荡30秒，甩去洗涤液，用吸水纸拍干。重复此操作4次。如果用洗板机洗涤，洗涤次数增加一次。
5. 每孔加入100ul的亲和链酶素-HRP，轻轻振荡混匀，37℃温育30分钟。
6. 甩去孔内液体，每孔加满洗涤液，振荡30秒，甩去洗涤液，用吸水纸拍干。重复此操作4次。如果用洗板机洗涤，洗涤次数增加一次。
7. 每孔加入底物A、B各50ul，轻轻振荡混匀，37℃温育5分钟。避免光照。
8. 取出酶标板，迅速加入50ul终止液，加入终止液后应立即测定结果。
9. 在450nm波长处测定各孔的OD值。
结果判断与分析：
1、 仪器值：于波长450nm的酶标仪上读取各孔的OD值
2、 以吸光度OD值为纵坐标（Y），相应的LTB4标准品浓度为横坐标（X），做得相应的曲线，样品的LTB4含量可根据其OD值由标准曲线换算出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。
人肠碱性磷酸酶(ALPI)ELISA检测试剂盒9017112 Kit, Service Prot. Plasticware, BR8 RD
9017091 Uprade kit, RoboVac base BR8 RD v4.2
9232335 Cable, pump, DISU, BR8000
9238771 Mission Pack Includes 6 working days (on-site or off-site work) by a QIAGEN Instrumentation Service Specialist, valid for 1 year. These days can be used for writing and/or optimization of protocols or for training. It includes all travel and labor charges. Parts, consumable chemistries, and accessories are not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed
9238919 Tooth belt 330mm, BS15
9238910 Disc tray, BS96
9238700 Front panel w. keyboard foil (Zent2)
9238693 Voltage regulator 7805 DC, 15 V
9232099 Front blind for size 120
9230968 Holder, X-drive motor, BR8000
9014724 DISU Retraction Mechanism BRMDx,complete
9014723 Scanner, Complete, SamTrack, BR8 RD
9014580 Vacuum Manifold, SBS
9014525 Modules, DISU, BR8 RD v1.0
9022576 Strut assembly, Hood, AS, right, Qsym
9022338 DVD, System Restore, DCU
9021982 Gas strut, door, QXA
9021614 Kit, upgrade QSAS Cabinet, Qsym
9021282 Drop catcher, waste chute, v1, Qsym
9238177 QIAplate Holder, silver
9240647 Internal BCR, MS-3 CCD, AP196/98
9240637 UPS, 240 Volt, AP
9240629 Supplemental Fuse Pack, AP196/98
9240618 Fuse, TE5, 1.6A, PCB, AP96/196
9240598 Brake Cylinder, AP96/196
9240588 Vacuum Switch Assy, Capper, AP96
9240578 Vacuum Switch Assy, Elevator, AP96
9240563 Fuse,PCB, 630, Eppendorf, AP196/98
9240526 Cable, Motor Axis #3, Capper I/O, AP98
9240500 Fluidic Valve, Reagent Pump, AP
9240240 Collet nut, TR
9240099 #Re-Installation, BR M48 The Re-Installation, BR M48 facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview. Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.
9239855 Compressor, v2.0
9019494 Measurement Gauge Kit, RCS
9238863 LiquiChip, Premium Agreement The Premium Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).
5000-1251 Motor Control PCB, DML2
5000-1513 H-Spring, DML2
6000-3119 Plate Washer I - Rinse Cap Assembly
5000-1417 Overlay 099A, VTX2
5050-1078 LIS Information Package
9240717 Lead Screw Assy, Axis 5, TR, AP
9240703 O-Ring, Valve, Reagent Pump, AP
9020806 Rotor Timing Calibration Dye, RGQ
338134 Microbial DNA Positive Control
288105 bactotype Mycoplasma Mg/Ms PCR Kit (96)
275002 flocktype NDV Ab (2)
285515 cador C. burnetii PCR Reagent (96)
285115 cador TKP PCR Reagent (96)
9001901 #QIAgility (PC) Priority QIAgility (PC) Priority includes the QIAgility instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor and travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
800331 PCR Amplification
9019489 ZLV PLATE ADAPTOR BIOHIT
9019544 ZLV Skirt, low raw white 2427, CASx
9019580 ZLV Reed Switch assembly, C34
9020583 X-Belt, DML3000 185 Teeth
9020592 Optical Head Assembly, DML 3000
9021015 Encoder, Z Axis, VR, DCU
9013209 Cabling kit, 5V/24V, BR8000
9013189 Wheel, retractable tubing, dispenser, BR
9011757 Reagent holder, 1-trough 20 ml Holder for accommodating a single disposable trough (20 ml) on the worktable; must be used with the reagent holder tray
132632 NeXtal Stock Potassium formate (50) 10M Potassium formate - 50mL (4 x 12,5 ml tube)
761125 PAXgene Blood DNA Tube (100) 100 PAXgene Blood DNA Tubes
