起订量:
SerpinB2抗体-纤溶酶原激活物抑制因子2/血浆酶原激活酶抑制因子-2抗体
免费会员
生产厂家上海研盟生物科技有限公司是一家专业化的免疫学产品研发及生产的国家*。主要为各大高校、医院、生物科研院所等提供*的科研抗体产品、重组蛋白技术、单/多克隆抗体定制生产及ELISA试剂盒定制开发和相关免疫技术服务。经过数年的努力发展已迅速成为集产品研发、生产、经营为一体的专业化生物工程公司。公司具有具有完善的实验技术开发平台,成熟的抗原、抗体研发系统,熟练掌握各种酶联技术,结合公司的研发团队,可以有效的保证公司产品强的稳定性和高的准确性,同时又具有Z有竞争力的价格。 公司目前可以提供生化试剂、分子生物学试剂及试剂盒,各种抗体及免疫学试剂盒、实验耗材等多门类上万种产品,产品涉及分子生物学、细胞生物学、免疫学等生命科学的各个领域。
销售热线:021-60895578
销售QQ:1017453315
上海研盟生物科技有限公司SerpinB2抗体-纤溶酶原激活物抑制因子2/血浆酶原激活酶抑制因子-2抗体*,主要应用于WB、IHC、IF、ELISA、流式细胞术等实验中。说明书随货发送,您也可以直接我司在线客服索取。客服
简单介绍:
中文名称:纤溶酶原激活物抑制因子2/血浆酶原激活酶抑制因子-2抗体
英文名称:Anti-SerpinB2
产品编号:BYK-1864R
产品别名:Serpin B2; Serpinb2; SerpinB2; plasminogen activator inhibitor 2; HsT1201; Monocyte Arg serpin; Monocyte Arg-serpin; PAI2A; PAI; PAI-2; PAI 2; PAI2; Placental plasminogen activator inhibitor; PAI2_RAT; PLANH2; Plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2 type A; Serpin peptidase inhibitor clade B (ovalbumin) member 2; Urokinase inhibitor.
产品规格:0.1ml、0.2ml、1ml
产品用途:科研实验
产品价格:请报价
说明书:请添加客服直接索取
抗体来源:该指标有两种产品,一种是兔来源抗体,一种是鼠来源抗体
克隆类型:兔来源为多克隆抗体,鼠来源单克隆抗体
交叉反应:请索取说明书查看
性 状:Lyophilized or Liquid
浓 度:1mg/1ml
亚 型:IgG
纯化方法: affinity purified by Protein A
储 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SerpinB2抗体-纤溶酶原激活物抑制因子2/血浆酶原激活酶抑制因子-2抗体产品介绍:SerpinB2 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in the placenta, and given the name PAI-2 because of the ability to inhibit urokinase plasminogen activator (u-PA) at low micromolar efficiency. SerpinB2 also inhibits tissue plasminogen activator (t-PA), but with micromolar efficiency, and PAI-1 is much more efficient that SerpinB2 on both forms of plasminogen activator. The structure of PAI-2 is not terribly similar to PAI-1, however, which is an E clade serpin.
SerpinB2 is made by many cell types, and is found intracellularly as an unglycosylated kDa protein, and secreted as a 60 kDa protein. SerpinB2 is found in saliva, secreted by gingival fibroblasts, and in the skin. SerpinB2 levels are elevated in serum during pregnancy, and in leukemia, breast cancer and ovarian cancer, although it was lowered in some cancers.A shorter SerpinB2 isoform of 382 amino acids, has been reported, with a predicted mass of 43.1 kDa and a pI of 5.69. The shorter form has a deletion just after the start of the mature protein, but it is unclear what the relative production and distribution is for the shorter sequence.
多肽的溶解与保存
一、冻干多肽的溶解
1、多肽溶解的主要问题是二级结构的形成。虽然疏水肽链二级结构的形成更明显,但除zui短的肽链外,此现象产生在几乎所有肽链,与极性无关。所以,溶解多肽的*个原则是使用无菌蒸馏水或去离子水,当然无氧水。多肽溶液可能遇到细菌降解,为防止此情况的发生,应溶解在无菌的蒸馏水中或用0.45或0.2孔径的滤膜过滤除菌。含有Cys、Met、Trp的多肽很容易氧化,应溶于无氧的水中,无氧水可通过注入惰性气体(氮、氦、氩)减压除气得到;
2、若多肽不溶于纯水,超声处理有助于打碎颗粒并增加溶解度。注意:超声处理会引起溶液发热或多肽降解;
3、若多肽含有多个碱性氨基酸,使用(1-10%)的乙酸水溶液:对于疏水性强的多肽,应使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸盐等挥发性的碱性缓冲液溶解。pH值在层析前必须调整;
5、异丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有机溶剂的量必须很少否则将严重影响停留时间;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香链而高度疏水、或为中性肽时,DMF或DMSO等膜变性剂的使用,有利于多肽的溶解:
a.高浓度模变性剂通过破坏多肽的二级结构而助溶;
b.膜变性剂适于多肽分析液的制备,但可能会对其生物活性的研究工作造成干扰;
c.DMF是变性剂(zui高浓度可达30%),滴加至多肽溶解;
d.反相层析法时,DMF将与洗脱液前锋一起流出,根据入量的多少,峰值可能很高。大多数肽能在大量DMF流出后几分钟内流出,若肽链很小,洗脱太早,多肽的量将很低。
相关产品:
钙粘蛋白相关的神经受体1(CNR-1)ELISA试剂盒,96T/48T科研实验
*加压素(AVP)ELISA试剂盒,96T/48T科研实验
毛细血管扩张性共济失调突变基因(ATM)ELISA试剂盒,96T/48T科研实验
酸性神经鞘磷脂酶(ASM)ELISA试剂盒,96T/48T科研实验
花生四烯酸5脂加氧酶(ALOX-5)ELISA试剂盒,96T/48T科研实验
5脂加氧酶(5-LO/LOX)ELISA试剂盒,96T/48T科研实验
芳香烃受体(AhR)ELISA试剂盒,96T/48T科研实验
晚期糖基化终末产物受体(RAGE/AGER)ELISA试剂盒,96T/48T科研实验
特异性巨噬细胞武装因子(SMAF)ELISA试剂盒,96T/48T科研实验
促有丝分裂因子(MF/MPF)ELISA试剂盒,96T/48T科研实验
腺苷酸环化酶1(AC-1)ELISA试剂盒,96T/48T科研实验
可溶性腺苷酸环化酶(sAC)ELISA试剂盒,96T/48T科研实验
抗鼠抗体(HAMA)ELISA试剂盒,96T/48T科研实验
钙离子通道抗体(VGCC)ELISA试剂盒,96T/48T科研实验
溴脱氧核苷*(BrdU)ELISA试剂盒,96T/48T科研实验
胃粘液素(GM)ELISA试剂盒,96T/48T科研实验
纤维母细胞表面抗原(FSP)ELISA试剂盒,96T/48T科研实验
转移因子(TF)ELISA试剂盒,96T/48T科研实验
*脱羧酶(ODC)ELISA试剂盒,96T/48T科研实验
黑色素瘤相关抗原(MAGE)ELISA试剂盒,96T/48T科研实验
前胶原C端肽酶(PCP)ELISA试剂盒,96T/48T科研实验
多形核白细胞弹性蛋白酶(PMN Elastase)ELISA试剂盒,96T/48T科研实验
补体片断3b(C3b)ELISA试剂盒,96T/48T科研实验
补体片断4b(C4b)ELISA试剂盒,96T/48T科研实验