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上海市所在地
大鼠β2-微球蛋白(β2M)ELISA Kit
面议二噁英检测试剂盒美国ABRaxis*
面议人脂肪细胞型脂肪酸结合蛋白(FABP4) ELISA Kit
面议elisa检测试剂盒
面议人B-细胞激活因子(BAFF)检测试剂盒
面议人细胞因子信号转导抑制因子3(SOCS3) ELISA Kit
面议G2(IgG2)检测试剂盒
面议人1(KLK1) ELISA Kit
面议大鼠*释放激素受体(GnRHR) ELISA Kit
面议小鼠转运蛋白1(TNPO1) ELISA Kit
面议大鼠AMP激活蛋白激酶β1(PRKAβ1) ELISA Kit
面议人黑素瘤细胞黏附分子(MCAM) ELISA Kit
面议猪链球菌(strep)检测试剂盒齐一生物科技(上海)有限公司试剂种类众多:覆盖分析化学品,无机化学品,有机化学品,生物化学品,材料化学品.试剂信息齐全:包含试剂特性描述,详细质量信息,应用方法、标准,用途以及正在开发的前沿应用.齐一生物科技(上海)有限公司:021- 60348496.www.qiyibio。。com
猪链球菌(strep)检测试剂盒一、高效、灵敏、特异的抗体;
二、稳定的重复性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相载体;
四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型;
五、节省实验经费.
elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度.比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%.
制备方法
包括以下步骤:
1)抗原表位的计算机筛选;
2)抗原的制备;
3)血清的采集;
4)间接ELISA方法的建立;
5)间接ELISA方法的敏感性和特异性验证;
6)试剂盒的稳定性验证;
7)对屠宰场进行临床检测.该方法简单、快速、灵敏度高、特异性强、稳定性合格、重复性好.应用本发明制得的试剂盒能有效检出感染猪的戊型肝炎病毒,适用于大批量发病样本的检测,可用于猪戊肝的快速诊断,并预防、控制猪戊肝病毒的流行和阻断戊肝病毒由猪向人传播.
组成结构
1、血清:操作过程中避免任何细胞刺激.使用不含热原和内毒素的试管.收集血液后,1000×g离心10分钟将血红细胞迅速小心地分离.
2、血浆:EDTA、柠檬酸盐、肝素血浆可用于检测.1000×g离心30分钟去除颗粒.
3、细胞上清液:1000×g离心10分钟去除颗粒和聚合物.
4、组织匀浆:将组织加入适量生理盐水捣碎.1000×g离心10分钟,取上清液.
5、保存:如果样品不立即使用,应将其分成小部分-70℃保存,避免反复冷冻.尽可能的不要使用溶血或高血脂血.如果血清中大量颗粒,检测前先离心或过滤.不要在37℃或更高的温度加热解冻.应在室温下解冻并确保样品均匀地充分解冻.
还原型谷胱甘肽+巯基测试盒/GSH+-SH测试盒
DAB浓缩型试剂盒
DNA产物纯化试剂盒
PCR纯化试剂盒
胶回收试剂盒
玻璃珠DNA胶回收试剂盒
EZ Spin柱式DNA胶回收试剂盒/UNIQ-10柱式DNA胶回收试剂盒/EZ Spin Column DNA Gel Extraction Kit
EZ Spin柱式PAGE胶DNA回收试剂盒/ UNIQ-10柱式PAGE胶DNA回收试剂盒/EZ Spin Column PAGE Gel DNA Extraction Kit
EZ Spin柱式PAGE胶Oligo DNA回收试剂盒/UNIQ-10柱式PAGE胶Oligo DNA回收试剂盒/EZ Spin Column PAGE Oligo Gel DNA Extraction Kit
EZ Spin柱式PCR产物纯化试剂盒/UNlQ-10柱式PCR产物纯化试剂盒/EZ Spin Column PCR Product Purification Kit
96孔PCR产物纯化试剂盒/96 well plate PCR Product Purification Kit
96孔DNA清洁试剂盒/96 well plate DNA Clean up Kit
玻璃珠PCR产物纯化试剂盒/Golden Beads PCR Product Purification Kit
EZ Spin柱式质粒小量抽提试剂盒/UNlQ-10柱式质粒小量抽提试剂盒/EZ Spin Column Plasmid Mini-Preps Kit
EZ Spin柱式质粒中量抽提试剂盒/UNlQ-200柱式质粒中量抽提试剂盒/EZ Spin Column Plasmid Medi-Preps Kit
EZ Spin柱式质粒大量抽提试剂盒/UNlQ-500柱式质粒大量抽提试剂盒/EZ Spin Column Plasmid Maxi-Preps Kit
96孔质粒DNA小量抽提纯化试剂盒/96 well plate Plasmid Mini-Preps Kit
96孔质粒DNA小量制备试剂盒/96 well plate preparation Mini-Preps Kit
EZ Spin柱式无内毒素质粒抽提试剂盒/UNlQ-500柱式无内毒素质粒抽提试剂盒/EZ Spin Column Endotoxin-free Plasmid Preps Kit
EZ Spin柱式一步式裂解质粒快速抽提试剂盒/UNlQ-10柱式一步式裂解质粒快速抽提试剂盒/EZ Spin Column one-step lysis Bacterial Plasmid Rapid Preps Kit
EZ Spin柱式酵母质粒抽提试剂盒/UNlQ-10柱式酵母质粒抽提试剂盒/EZ Spin Column Yeast Plasmid Preps Kit
EZ Spin柱式细菌基因组DNA抽提试剂盒/UNlQ-10柱式细菌基因组DNA抽提试剂盒/EZ Spin Column Bacterial Genomic DNA Isolation Kit
96孔细菌基因组DNA小量抽提试剂盒/96 well plate Bacterial Genomic DNA Mini-Preps Kit
Mouse hypoxia-inducible factor 1α,HIF-1α ELISA Kit
Mouse Neonatal Thyroxine,NN-T4 ELISA Kit
Mouse Amylin ELISA Kit
Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit
Mouse Leukotriene C4,LT-C4 ELISA Kit
Mouse Cytochrome-C,Cyt-C ELISA Kit
Mouse Free Thyroxine,FT4 ELISA Kit
Mouse angiotension Ⅰ,Ang-Ⅰ ELISA Kit
Mouse melanocyte antibody,MC Ab ELISA Kit
Mouse thyroxine,T4 ELISA Kit
Mouse dipeptidyl peptldase Ⅳ,DPPⅣ ELISA Kit
Mouse Complement fragment 4a,C4a ELISA Kit
Mouse Tri-iodothyronine,T3 ELISA Kit
Mouse Complement fragment 5a,C5a ELISA Kit
Mouse Complement fragment 3a,C3a ELISA Kit
Mouse 17-Hydroxyprogesterone,17-OHP ELISA Kit
Mouse Follistatin,FS ELISA Kit
Mouse Histone Deacetylase,HD ELISA Kit
Mouse Estriol,E3 ELISA Kit
Mouse Androstenedione,ASD ELISA Kit
Mouse Free Testoterone,F-TESTO ELISA Kit
Mouse Testoterone,T ELISA Kit
Mouse Progesterone,PROG ELISA Kit
Mouse soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit
Mouse Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit
Mouse Endothelin 1,ET-1 ELISA Kit
Mouse Estradiol,E2 ELISA Kit
Mouse Thyroid Stimulating Hormone,TSH ELISA Kit
Mouse Methylase ELISA Kit
Mouse proliferating cell nuclear antigen antibody,PCNA ELISA Kit
Mouse lipoprotein lipase,LPL ELISA Kit
Mouse tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Mouse Glucose-dependent insulin-releasing polypeptide,GIP ELISA Kit
Mouse Glycogen phosphorylase II,GP-II ELISA Kit
Mouse Glycogen phosphorylase MM,GP-MM ELISA Kit
Mouse Glycogen phosphorylase BB,GP-BB ELISA Kit
Mouse Thyroxine antibody,TAb ELISA Kit
Mouse thyroid stimulating hormone receptor antidoby,TRAb ELISA Kit
Mouse catecholamine,CA ELISA Kit
Mouse Interleukin 27,IL-27 ELISA Kit
Mouse anti-alpha-Fodrin IgGIgA,α-Fodrin IgGIgA ELISA Kit
Mouse lipoprotein α,Lp-α ELISA Kit
Mouse Big Endothelin,Big ET ELISA Kit
Mouse coagulation factor Ⅷ related antigen,FⅧ-Ag ELISA Kit
Mouse Fcoagulation factor Ⅸ,FⅨ ELISA Kit
Mouse coagulation factor Ⅹ,FⅩ ELISA Kit
Mouse von Willebrand Factor,vWF ELISA Kit
Mouse Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit
Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT
Mouse plasmin-antiplasmin complex,PAP ELISA Kit
Mouse thrombin-antithrombin complex,TAT ELISA Kit
Mouse anti-thrombin receptor,ATR ELISA Kit
Mouse thrombin receptor,TR ELISA Kit
Mouse cardiac troponin Ⅰ,cTn-Ⅰ ELISA Kit
Mouse Phosphotylinosital 3 kinase,PI3K ELISA Kit
mouse Beta-Endorphin,β-EP ELISA Kit
Mouse Ischemia Modified Albumin,IMA ELISA Kit
Mouse protein kinase B,PKB ELISA Kit