转铁蛋白受体抗体Transferrin receptor英文名称    Transferrin receptor
中文名称    转铁蛋白受体抗体
别    名    CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; protein 1, serum form; TRFR.
研究领域    肿瘤  心血管  细胞生物  免疫学  神经生物学  干细胞  糖尿病  细胞表面分子  细胞类型标志物  淋巴细胞  t-淋巴细胞  b-淋巴细胞  
抗体来源    Rabbit
克隆类型    Polyclonal
交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    74/85kDa
细胞定位    细胞膜 
性    状    Lyophilized or Liquid
浓    度    1mg/1ml
转铁蛋白受体抗体Transferrin receptor免 疫 原    KLH conjugated synthetic peptide derived from human CD71
亚    型    IgG
纯化方法    affinity purified by Protein A
储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍    background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.

Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. 
Transferrin receptor protein 1, serum form: Secreted

Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. 
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). 
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Similarity:
Belongs to the peptidase M28 family. M28B subfamily. 
Contains 1 PA (protease associated) domain.

Database links:
Entrez Gene: 7037 Human
Entrez Gene: 22042 Mouse
Entrez Gene: 64678 Rat
Omim: 190010 Human
SwissProt: P02786 Human
SwissProt: Q62351 Mouse
SwissProt: Q99376 Rat
Unigene: 529618 Human
Unigene: 28683 Mouse
Unigene: 98672 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

转铁蛋白及转铁蛋白受体(TfnrR)是机体铁代谢中的重要因子，铁是细胞生长增殖、代谢的必需元素,转铁蛋白受体(TfR)介导的胞吞作用是细胞摄取铁的主要途径。肿瘤细胞因增殖生长旺盛,对铁的需求高,所以肿瘤细胞有比正常细胞更多的转铁蛋白受体的表达。