Streptavidin,Cy3标记链霉亲和
时间:2015-02-09 阅读:578
Streptavidin,Cy3标记链霉亲和素 conjugated
Catalog Number : RS-0437P-cy3
Quantity size : 0.1ml (1mg/ml; Buffer = 0.01M PBS, pH 7.4 with 1% BSA,0.03% Proclin300 and 50% Glycerol. )
Background: Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Key Applications :
· FCM: 1:20-100
· Immunohistochemistry : 1:20-200
· Immunocytochemistry: 1:20-200
· Excitation spectrum: 530-550nm
· Emission spectrum: 560-570nm
· not yet tested in other applications.
· Optimal working dilutions must be determined by the end user.
Species Reactivity: Streptavidin
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.