细胞名称

EDJ#22小鼠胚胎干细胞EDJ#22（干细胞库保藏）

描述

小鼠*性胚胎干细胞，每支细胞量1×10⁶

动物种别

小鼠，129S5/SvEvTac品系

性别

雄

组织来源

小鼠胚胎内细胞团

形态

球形克隆

培养基和添加剂

mES*培养液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（终浓度为1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。

供应限制

REFERENCE

Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999. PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Threadgill DW, et al. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome. 8(6):390-393 1997. PubMed: 9166580 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865