EliKine™ Human CRP ELISA Kit

Human

ELISA

EliKine™ Human CRP ELISA Kit employs a two-site sandwich ELISA to quantitate CRP in samples. An antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRP is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Colorimetric

Cell culture supernatants, Other biological fluids, Plasma, Serum

Sandwich ELISA (quantitative)

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.