细胞名称

OP9小鼠骨髓基质细胞

描述

OP9细胞株源自新生的op/op 小鼠颅盖。因编码M-CSF的基因中的一个突变，它不能生成有功能的巨噬细胞克隆刺激因子(M-CSF)。M-CSF的存在对胚胎干细胞(ES)分化成血细胞而不是其他巨噬细胞有抑制功能。 OP9细胞可以用于与小鼠胚胎干细胞共培养以诱导胚胎干细胞分化成成红血球来源的、骨髓来源的和B细胞谱系的血细胞。与OP9共培养不需要外源的生长因子或复杂的胚胎结构。这个系统对研究造血细胞的发育和分化的分子机理有用。 在本库通过支原体检测。

动物种别

小鼠

性别

***

组织来源

骨髓，基质

形态

成纤维细胞

培养基和添加剂

MEMα-培养基(GIBCO,添加NaHCO3 1.5g/L，肌醇 43.2mg/L, * 8.82mg/L,β-巯基乙醇 7.8mg/L)，80%；优质胎牛血清，20%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

供应限制

REFERENCE

Nakano T , et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265: 1098-1101, 1994. PubMed: 8066449 Nakano T , et al. In vitro development of primitive and definitive erythrocytes from different precursors. Science 272: 722-724, 1996. PubMed: 8614833 Nakano T . Lymphohematopoietic development from embryonic stem cells in vitro. Semin. Immunol. 7: 197-203, 1995. PubMed: 7579206 Motoyama N , et al. bcl-x prevents apoptotic cell death of both primitive and definitive erythrocytes at the end of maturation. J. Exp. Med. 189: 1691-1698, 1999. PubMed: 10359572 Nakano T . In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis. Int. J. Hematol. 65: 1-8, 1996. PubMed: 8990620 Nakano T , et al. Development of erythroid cells from mouse embryonic stem cells in culture: potential use for erythroid transcription factor study. Leukemia 3: 496-500, 1997. PubMed: 9209437 Suwabe N , et al. GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation. Blood 92: 4108-4118, 1998. PubMed: 9834216 Suzuki A , Nakano T . Development of hematopoietic cells from embryonic stem cells. Int. J. Hematol. 73: 1-5, 2001. PubMed: 11372743 Eto K , et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. USA 99: 12819-12824, 2002. PubMed: 12239348

中文名称