细胞名称

RAG小鼠肾腺癌细胞

描述

这是一株肾脏-2α细胞株的非回复8-氮杂*抗性(HPRT-, HGPRT-)克隆。 测试发现缺肢畸形病毒(鼠痘)阴性。 在本库通过支原体检测。

动物种别

小鼠

性别

***

组织来源

肾腺癌；肾

形态

变形虫样

培养基和添加剂

MEM培养基(GIBCO,添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

供应限制

REFERENCE

Klebe RJ , et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091 Science 145: 709, 1964. Klebe RJ , et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843 Felluga B , et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. PubMed: 5797837 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

中文名称