细胞名称

C57BL/6小鼠胚胎干细胞C57BL/6

描述

小鼠*性胚胎干细胞，每支细胞量1×10⁶

动物种别

小鼠，C57BL/6品系

组织来源

小鼠胚胎内细胞团

形态

球形克隆

培养基和添加剂

mES*培养液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（终浓度为1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。

供应限制

REFERENCE

Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514 Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 Schuster-Gossler K, et al. Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques 31: 1022-1024, 1026, 2001. PubMed: 11730008 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach.Oxford, Oxford University Press, 101-132, 1999 Lemckert FA, et al. Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res. 25: 917-918, 1997. PubMed: 9016649 Ledermann B, Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp. Cell Res. 197: 254-258, 1991. PubMed: 1959560

中文名称