胰岛素样生长因子1(IGF1)多克隆抗体

Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

胰岛素样生长因子1(IGF1)多克隆抗体IMMUNOGEN INFORMATION

Immunogen: Recombinant IGF1 (Gly33~Ala102) expressed in E.coli.

ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IGF1. It has been selected for its ability to recognize IGF1 in immunohistochemical staining and western blotting.

APPLICATIONS

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

胰岛素样生长因子1(IGF1)多克隆抗体CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.

IHC Images

Used in DAB staining on fromalin fixed paraffin-embedded liver tissue

QUALITY CONTROL

Content: The quality control contains recombinant IGF1 (Gly33~Ala102) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
胰岛素样生长因子1(IGF1)多克隆抗体Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

胰岛素样生长因子1(IGF1)多克隆抗体STORAGE

Store at 4^oC for frequent use. Stored at -20^oC to -80^oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.