产品简介
重组多磷酸激酶/PPK 产品编号：ENZ-266
产品规格：50IU/100IU/200IU
详细介绍
重组多磷酸激酶/PPK
产品编号：ENZ-266
产品规格：50IU/100IU/200IU
| Catalogue Number | ENZ-266 | | Synonyms | Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase. | | Introduction | PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction. | | Description | Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD （monomer）. | | Source | Propionibacterium shermanii. | | Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | | Formulation | The protein was lyophilized from 1.54ml PPK solution containing 32.45U/ml of PPK activity, 3.87mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate. | | Solubility | The contents of the vial can be reconstituted in 1-10 ml deionized water. | | Stability | Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. | | Definition of Activity Units | The amount of Polyphosphate kinase required to convert 1 μmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor. | | Specific Activity | 8.39 U/mg. | | Required Cofactors | The reaction requires Mg2+. | | Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | | |
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