【文献标题】iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae

【作者】Rining Zhu, Chuntong Baoet.al

【作者单位】吉林大学（ Jilin University）

【文献中引用产品】

猪甘露糖受体C1样蛋白1(MRC1)ELISA试剂盒

猪过氧化物酶2（PRDX2）ELISA试剂盒

猪补体因子B(CFB)ELISA

猪整合素β（ITG-β）ELISA试剂盒

猪E钙粘附分子(E-Cadherin)ELISA试剂盒

【关键词】A. pleuropneumoniae, iTRAQ, Porcine pleuropneumonia, Comparative proteomics, Serum

【DOI】doi.org/10.1186/s13568-020-01057-9

【影响因子(IF)】4.12

【出版期刊】《AMB Expr》

【产品原文引用】Five proteins were selected for validation by ELISA:MRC1,PRDX2,CFB,ITGB and E-Cadherin. Te serum samples were allowed to clot for 1h at room temperature and then separated by centrifugation at 2000×g for 

15min at 4?°C. Te serum was then frozen until analyzed by ELISA. Te concentration of MRC1,PRDX2,CFB,ITGB and E-Cadherin was determined using commercial porcine ELISA kits (Shanghai Jinma Biological Technology, Inc., China) according to the manufacturer’s instructions. All samples were analyzed in duplicate. Cytokine concentrations were calculated using linear regression analysis based on the OD of the cytokine standards provided by the manufacturer.

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