【文献标题】Treatment of corn with lactic acid or hydrochloric acid modulates the rumen and plasma metabolic profiles as well as inflammatory responses in beef steers

【作者】You Yang、Guozhong Dong、 Zhi Wang、,Junhui Liu et.al

【作者单位】 Southwest University（西南大学）

【文献中引用产品】

牛白细胞介素1β（IL-1β）ELISA试剂盒

牛白细胞介素6(IL-6)ELISA试剂盒

牛白细胞介素8（IL-8）ELISA试剂盒

牛肿瘤坏死因子α（TNF-α）ELISA试剂盒

【关键词】Metabolomics, Beef steers, Lactic acid, Hydrochloric acid, Corn

【DOI】10.1186/s12917-018-1734-3

【影响因子(IF)】2.6

【出版期刊】《BMC Veterinary Research 》

【产品原文引用】

The LPS concentrations in the rumen fluid and plasma;the concentrations of acute phase proteins in plasma,including C-reactive protein (CRP), serum amyloid A(SAA), haptoglobin (Hp), and LPS-binding protein(LBP); and plasma cytokines concentrations, including interleukin (IL)-1β, IL-6, IL-8 and tumour necrosis  factor-alpha (TNF-α), were determined using a commercially available bovine ELISA kit. A bovine LPS  ELISA kit was purchased from Shanghai Preferred Biotechnology Co., Ltd. (Shanghai, China). Bovine SAA,CRP, Hp and LBP ELISA kits were purchased from Nanjing Jianchen Saihao Biotechnology Co., Ltd. (Nanjing, China). Bovine IL-1β, IL-6, IL-8 and TNF-α ELISA  kits were purchased from Shanghai Jinma Biotechnology Co., Ltd. (Shanghai, China). According to the manufacturer’s instructions, all samples were tested in  duplicate, and the optical density values were read at  450 nm on an automatic microplate reader (EIX808IU;Biotek, Winooski, VT).

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