大鼠中性粒细胞弹性蛋白酶（NE）酶联免疫分析(ELISA)试剂盒

实验目的：本试剂盒仅供研究使用，用于测定大鼠血清，血浆及相关液体样本中中性粒细胞弹性蛋白酶（NE）含量。

实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠中性粒细胞弹性蛋白酶（NE）水平。用纯化的大鼠中性粒细胞弹性蛋白酶（NE）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入中性粒细胞弹性蛋白酶（NE），再与HRP标记的中性粒细胞弹性蛋白酶（NE）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的中性粒细胞弹性蛋白酶（NE）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠中性粒细胞弹性蛋白酶（NE）浓度。

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.92以上。

2.批内与批见应分别小于9%和15%

Drug Names

Generic Name：Rat neutrophil elastase (NE) ELISA Kit.

Purpose

This kit allows for the determination of NE concentrations in Rat serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Rat NE level in the sample，use Purified Rat NE antibody to coat microtiter plate wells, make solid-phase antibody, then add NE to wells, Combined NE which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.