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大鼠*Ⅹ酶联免疫试剂盒
面议大鼠复合前列腺特异性抗原(CPSA)elisa试剂盒
面议大鼠磷脂酰肌醇抗体IgG/IgM(PI Ab-IgG/IgM)elisa试剂盒
面议大鼠抗中性粒/中心体抗体(ACA)elisa试剂盒
面议大鼠抗心肌抗体(AMA)elisa试剂盒
面议大鼠促黄体激素(LH)elisa试剂盒
面议大鼠绒毛膜*(CG)elisa试剂盒
面议大鼠C肽(C-Peptide)elisa试剂盒
面议大鼠胰岛素(INS)elisa试剂盒
面议大鼠新生甲状腺素(NN-T4)elisa试剂盒
面议大鼠游离三碘甲状腺原氨酸(Free-T3)elisa试剂盒
面议大鼠游离甲状腺素(FT4)elisa试剂盒
面议人脂质运载蛋白2(LCN2)ELISA Kit
Human lipocalin 2,LCN2 ELISA Kit
样本处理及要求:
血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
人脂质运载蛋白2(LCN2)ELISA Kit性能:
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批间应分别小于9%和15%
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
ELISA试剂盒供应商:上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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