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人乙型肝炎表面抗原酶联免疫检测试剂盒
面议人基质金属蛋白酶组织抑制因子1(TIMP-1)elisa试剂盒
面议人Ⅳ型胶原(Col Ⅳ)elisa试剂盒
面议人颗粒酶B(Gzms-B)elisa试剂盒
面议人醛缩酶酶联免疫检测试剂盒,人醛缩酶elisa检测试剂盒
面议人可溶性血管内皮细胞蛋白C受体(sEPCR)elisa试剂盒
面议人8羟基脱氧鸟苷(8-OHdG)elisa试剂盒
面议人白介素2受体酶联免疫检测试剂盒,人白介素2受体elisa检测试剂盒
面议人叉头框蛋白04(FoxO4)elisa试剂盒
面议大鼠*Ⅹ酶联免疫试剂盒
面议大鼠复合前列腺特异性抗原(CPSA)elisa试剂盒
面议大鼠磷脂酰肌醇抗体IgG/IgM(PI Ab-IgG/IgM)elisa试剂盒
面议HE037
豚鼠神经肽B(NKB)ELISA试剂盒
Guinea pig Neurokinins B,NKB ELISA Kit
样本处理方法:
血清样本:室温血液自然凝固10-20分钟,离心 20 分钟左右(2000-3000转/分)。仔细收集
上清,保存过程中如出现沉淀,应再次离心。
血浆样本:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心
20 分钟左右(2000-3000 转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次
离心。
尿液样本:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程
中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
豚鼠神经肽B(NKB)ELISA试剂盒细胞培养上清样本:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000 转/
分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞
浓度达到100万/ml 左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分
钟左右(2000-3000 转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
组织标本:切割标本后,称取重量。加入一定量的 PBS,PH7.4。用液氮迅速冷冻保存备
用。标本融化后仍然保持2-8℃的温度。加入一定量的 PBS(PH7.4),用手工或匀浆器
将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待
检测,其余冷冻备用。
标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上
进行试验,可将标本放于-20℃保存,但应避免反复冻融,不能检测含 NaN3 的样品,因
NaN3抑制辣根过氧化物酶的(HRP)活性。
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at
the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared,
Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20
mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,
If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of
2000-3000 r.p.m.remove supernatant, If precipitation appeared, Centrifugal again.
The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile
container,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove
supernatant,detect the composition of cells, Dilut cell suspension with PBS
(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw
cycles, damage cells and release of intracellular components, centrifugation 20-
min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation
appeared, Centrifugal again.
5. Tissue samples-After cutting samples, check the weight,add PBS(PH7.2-7.4),
Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add
PBS(PH7.4),Homogenized by hand or Grinders, centrifugation 20-min at the speed
of 2000-3000 r.p.m.remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the
relevant literature, and should be experiment as soon as possible after the
extraction. If it can’t,specimen can be kept in -20 ℃ to preserve, Avoid
repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
供应商:上海华壹生物科技有限公司