Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain （IL-2Ra）. CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 （IL-2 receptor beta chain） and CD132 （common γ chain） to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody （typically PC61.5） and subsequent detection with another CD25 antibody.