CHO-K1细胞,仓鼠卵巢细胞亚株

CHO-K1细胞,仓鼠卵巢细胞亚株

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CHO-K1细胞,仓鼠卵巢细胞亚株
ATCC来源,上海博研生物科技有限公司拥有完善的售前、售后服务,提供,售出细胞出现任何质量问题可免费退换,咨询:

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描述:该细胞株是源自成年中国仓鼠卵巢深入活体组织切片的CHO细胞的亚克隆,已通过STR检测。 已通过支原体检测。

产品名称:CHO-K1细胞,仓鼠卵巢细胞亚株

产品简介:ATCC来源,上海博研生物科技有限公司拥有完善的售前、售后服务,提供,售出细胞出现任何质量问题可免费退换,咨询:。
产品库存:现货
产品价格:询价
供 应 商:ATCC

动物种别:仓鼠

组织来源:卵巢

形态:上皮细胞

 

CHO-K1细胞,仓鼠卵巢细胞亚株培养基和添加剂:F12K培养基(SIGMA,货号N3520,添加NaHCO3 2.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

技术相关问答:
1. 如何选用特殊细胞系培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,*MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养是一个好的开始。2. 何时须更换培养基?视细胞生长密度而定, 或遵照细胞株基本数据上之更换时间,按时更换培养基即可。 3. 可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其特定使用且已适应之细胞培养基, 若骤然使用和原先提供之培养条件不同之培养基, 细胞大都无法立即适应, 造成细胞无法存活。4.可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个极为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生*的影响。来自不同物种的血清, 在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活。基本共性:
1、所有的细胞表面均有由磷脂双分子层与镶嵌蛋白质及糖被构成的生物膜,即细胞膜。
2、所有的细胞都含有两种核酸:即DNA与RNA。
3、作为遗传信息复制与转录的载体。
4、作为蛋白质合成的机器—核糖体,毫无例外地存在于一切细胞内,核糖体,是蛋白质合成的机器,在细胞遗传信息流的传递中起重要作用。
5、所有细胞的增殖都以一分为二的方式进行分裂。
6、能进行自我增殖和遗传
7、新陈代谢
8、细胞都具有运动性,包括细胞自身的运动和细胞内部的物质运动

 

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