The 0F' genotype is identical to 0 with the addition of an F' episome. The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' carries the lacI^q repressor for inducible expression from trc, tac, and lac promoters using IPTG. 0F' require IPTG induction for blue/white color screening.

The 0F' genotype offers the following features:

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacI^q repressor for inducible expression from trc, tac, and lac promoters

Note: This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F'{lacI^qTn10(Tet^R)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG