Use PRO Kit for identification of Candida albicans and Clostridium difficile. C. albicans is the yeast pathogen most frequently isolated from clinical specimens¹. In 1990, Perry et al. evaluated a rapid test to aid in the identification of C. albicans based on colorimetric detection of the enzyme, L-proline aminopeptidase². C. difficile is recognized as the major cause of antibiotic-associated pseudomembranous colitis and a frequent cause of hospital-acquired diarrhoea. Fedorko and Williams reported detection of L-Proline aminopeptidase is useful for identification of C. difficile because it is one of the few biochemical tests for which this organism is positive³.

• Easy to store - No special storage required
• Easy to read - After addition of one drop of Cinnamaldehyde a color change from red to pink color development indicates a positive test

The PRO disk contains the substrate L-proline-β-naphthylamide. This substrate is hydrolyzed by an aminopeptidase releasing β-naphthylamine. After addition of Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ- dimethyl-aminocinnamaldehyde to form a red-colored complex.