InvitrogenCellBlox™ Plus Blocking Buffer
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厂商性质
所在地
EVOS™ 台式容器支架,一个 Nunc™ 多孔培养板
面议Olympus™ 60X 油镜,X-Apo,1.42NA/0.15WD
面议EVOS™ 台式容器支架、一个 25 mL 烧瓶
面议Olympus 2X 物镜,复消色差透镜,LWD
面议EVOS™ Onstage Vessel Holder, one 75 x 50 mm microscope slide
面议EVOS™ 光立方,白色
面议Pierce™ 免疫染色增强剂
面议Arcturus Cellect 激光捕获显微切割系统
面议FluoSpheres™ 多尺寸规格试剂盒 #2,羧基修饰微球,黄绿色荧光 (505/515),2% 固体,六种规格
面议Fluoro-Max 绿色和红色干性荧光微粒
面议FluoSpheres™ 聚苯乙烯微球,10 µm,黄绿色荧光 (505/515),用于血流测定
面议FluoSpheres™ 羧基修饰微球
面议Invitrogen CellBlox Plus Blocking Buffer is formulated to block nonspecific binding of NovaFluor labels with cells. These nonspecific interactions can result in higher background labeling. CellBlox Plus Blocking Buffer is a non-antibody blocking solution and should be used whenever a NovaFluor dye is used for labeling of any cell type. CellBlox Plus Blocking Buffer has enhanced blocking capabilities compared to our original CellBlox Blocking Buffer (Figure 1).
CellBlox Plus Blocking Buffer is also recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling (Figure 2).
Features of CellBlox Plus Blocking Buffer include:
• Compatible with all fluorophores and with LIVE/DEAD Fixable dead cell stains
• Can be used with any fluorophore-antibody conjugate as high-performance monocyte and macrophage blocking solution
• Compatible with other blocking reagents, such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer
• Not required when labeling antibody-capture beads, such as UltraComp eBeads Plus Compensation Beads
Use of CellBlox Plus Blocking Buffer requires minimal change to most flow cytometry staining protocols. Simply add CellBlox Plus Blocking Buffer to an antibody cocktail mixture prior to labeling cells. Specifically, add 5 µL CellBlox Plus Blocking Buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 µL as a final staining volume.