上海研盟生物科技有限公司Integrin alpha 1抗体-整合素α1抗体*，主要应用于WB、IHC、IF、ELISA、流式细胞术等实验中。说明书随货发送，您也可以直接我司在线客服索取。客服

简单介绍：

中文名称：整合素α1抗体

英文名称：Anti-Integrin alpha 1

产品编号：BYK-2095R

产品别名：CD 49a; CD49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1.           

产品规格：0.1ml、0.2ml、1ml

产品用途：科研实验

产品价格：请报价

说明书：请添加客服直接索取

抗体来源：该指标有两种产品，一种是兔来源抗体，一种是鼠来源抗体

克隆类型：兔来源为多克隆抗体，鼠来源单克隆抗体

交叉反应：请索取说明书查看

性    状：Lyophilized or Liquid

浓    度：1mg/1ml

亚    型:IgG

纯化方法: affinity purified by Protein A

储 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Integrin alpha 1抗体-整合素α1抗体产品介绍：Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen.

多肽的溶解与保存

一、冻干多肽的溶解

1、多肽溶解的主要问题是二级结构的形成。虽然疏水肽链二级结构的形成更明显，但除zui短的肽链外，此现象产生在几乎所有肽链,与极性无关。所以，溶解多肽的*个原则是使用无菌蒸馏水或去离子水，当然无氧水。多肽溶液可能遇到细菌降解，为防止此情况的发生，应溶解在无菌的蒸馏水中或用0.45或0.2孔径的滤膜过滤除菌。含有Cys、Met、Trp的多肽很容易氧化，应溶于无氧的水中，无氧水可通过注入惰性气体（氮、氦、氩）减压除气得到；

2、若多肽不溶于纯水，超声处理有助于打碎颗粒并增加溶解度。注意:超声处理会引起溶液发热或多肽降解；

3、若多肽含有多个碱性氨基酸，使用（1-10%）的乙酸水溶液：对于疏水性强的多肽，应使用50%的乙酸；

4、若多肽中含有大量酸性氨基酸，可用氨溶液（1-10%）或乙酸乙基*或重碳酸盐等挥发性的碱性缓冲液溶解。pH值在层析前必须调整；

5、异丙醇和乙腈能溶解中等大小的多肽。若肽要上柱，有机溶剂的量必须很少否则将严重影响停留时间；

6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香链而高度疏水、或为中性肽时，DMF或DMSO等膜变性剂的使用，有利于多肽的溶解：

a.高浓度模变性剂通过破坏多肽的二级结构而助溶；

b.膜变性剂适于多肽分析液的制备，但可能会对其生物活性的研究工作造成干扰；

c.DMF是变性剂（zui高浓度可达30%），滴加至多肽溶解；

d.反相层析法时，DMF将与洗脱液前锋一起流出，根据入量的多少，峰值可能很高。大多数肽能在大量DMF流出后几分钟内流出，若肽链很小，洗脱太早，多肽的量将很低。

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