Iodoacetyl-LC-Biotin,93285-75-7,生物素乙酰碘交联剂

Iodoacetyl-LC-Biotin

Iodoacetyl-LC-Biotin (N-Iodoacetyl-N'-Biotinylhexylenediamine or N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine) reacts with sulfhydryl groups to form stable thioether linkages. Iodoacetyl-LC-Biotin has an extended spacer arm that reduces steric hindrance. Iodoacetyl-LC-Biotin must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous biotinylation reaction. Iodoacetyl-LC-Biotin coupling reactions are most efficient when reacted at at pH 7.5 - 8.5 in reduced light. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin or Immobilized Streptavidin.

Spacer Length: 27.1 Å 
Molecular Weight: 510.43 
CAS Number:93285-75-7 

Alternative Names: N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine; N-iodoacetyl-N-biotinylhexylenediamine (IAB); Iaa-Biotin 

结构式：

Sulfo-NHS-SS-Biotin

Sulfo-NHS-SS-Biotin biotinylation reagent, Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is cleavable, water-soluble and amino-reactive. The Sulfo-NHS-SS-Biotin molecule is cell membrane impermeable due to its sodium sulfonate group. Sulfo-NHS-SS-Biotin has a long chain spacer arm separating the biotin group from the amino-reactive sulfo-NHS ester moity. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin. It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of 2-mercaptoethanol, TCEP-HCl or DTT.

Molecular Weight: 606.69 
Spacer Length: 24.3 Å 
CAS Number:325143-98-4 

Alternative Names: Biotin disulfide N-Hydroxysulfosuccinimide ester; Sulfo NHS SS Biotin 

结构式;

Biotin-HPDP

N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)-propionamide (Biotin-HPDP) is a sulfhydryl reactive biotinylation reagent with a long chain, cleavable spacer arm. Biotin-HPDP biotinylation occurs between pH 6-9 through its pyridyldithiol group reacting with sulfhydryls to form a stable disulfide bond. The 29.2 angstrom spacer arm prevents steric hindrance and may also be cleaved at the disulfide bond using TCEP-HCl, DTT or ß-Mercaptoethanol. Biotin-HPDP must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous biotinylation reaction. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin or Immobilized Streptavidin.

Spacer Length: 29.2 Å 
Molecular Weight: 539.78 
CAS Number:129179-83-5 

Alternative Names: Biotin HPDP; Pyridyldithiol-biotin; Pyridyldisulfide-biotin 

结构式：

Sulfo-NHS-LC-Biotin

Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can biotinylate intracellular proteins. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin. 

The "long chain" Sulfo-NHS-LC-Biotin and Sulfo-NHS-Biotin both have the same chemical functionality. However, Sulfo-NHS-LC-Biotin will provide a longer spacer arm that is designed to overcome any steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg). Often, the best results are determined by empirically testing each reagent for your specific application.

Molecular Weight: 556.6 
Spacer Length: 22.4 Å 
CAS Number:127062-22-0 

Alternative Names: Biotin-X-NHS, Water-Soluble; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfo NHS LC Biotin; sulfosuccinimidyl biotinamido hexanoate; sulfo succinimidyl biotinamido hexanoate; Biotinamidocaproate N-hydroxysulfosuccinimidyl ester; sulfosuccinimidyl biotinamidyl hexanoate; long chain Sulfo NHS Biotin 

结构式：

NHS-SS-Biotin

NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced. NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester). The resulting biotinylated protein can then be detected when used in conjunction with streptavidin. 

It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), TCEP-HCl or DTT. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.

Molecular Weight: 504.65 
Spacer Length: 24.3 Å 
CAS Number:142439-92-7 

Alternate Names: Biotin disulfide N-Hydroxysuccinimide ester; NHS SS Biotin 

结构式：

Sulfo-NHS-Biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. It is amine reactive and has a spacer arm length of 13.5 Å. The Sulfo-NHS-Biotin biotinylation reagent is ideal for biotinylating antibody or protein that can then be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. The water insoluble version of Sulfo-NHS-Biotin is NHS-Biotin. 

Sulfo-NHS-Biotin and the "long chain" Sulfo-NHS-LC-Biotin both have the same chemical functionality, but the latter will provide a longer spacer arm more capable of overcoming steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg). Often, the best results are determined by empirically testing each reagent for your specific application.

Molecular Weight: 443.4 
Spacer Length: 13.5 Å 
CAS Number:119616-38-5 

Alternative Names: Biotin-NHS, Water-Soluble; Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfosuccinimidobiotin; Sulfo NHS Biotin; Sulfosuccinimidyl biotin; N-hydroxysulfosuccinimidyl biotin; N-hydroxysulfosuccinimido biotin 

结构式;

NHS-Biotin

NHS-Biotin (Succinimidyl Biotin; Succinimidobiotin) is a primary amine reactive biotinylation reagent used in Biotin-Streptavidin binding reactions. The NHS-Biotin molecule is cell membrane permeable and can be used to covalently label intracellular proteins and is often used in protein detection when used in conjunction with streptavidin and Immobilized Streptavidin for various Biotin-Streptavidin binding assays. The water soluble version of NHS-Biotin is Sulfo-NHS-Biotin.

Molecular Weight: 341.38 
Spacer Length: 13.5 Å 
CAS Number:35013-72-0 

Alternative Names: Succinimidobiotin; Biotin-OSU; Biotinyl-N-hydroxysuccinimide ester; Biotin N-hydroxysuccinimide ester; (+)-Biotin N-succinimidyl ester; NHS Biotin; succinimidiylbiotin; N-hydroxysuccinimide biotin; hydroxysuccinimide biotin; succinimidylbiotin; Biotin NHS; Biotin-NHS; N-Hydroxysuccinimidyl biotin; N-Hydroxysuccinimido biotin 

结构式：

NHS-LC-Biotin

NHS-LC-Biotin, Succinimidyl 6-(biotinamido)hexanoate, is an amino-reactive biotinylation reagent with a long spacer arm. The 6-atom spacer arm of NHS-LC-Biotin provides distance between the biotin and the target ligand which helps alleviate steric hindrance. NHS-LC-Biotin is used to biotinylate proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The NHS-LC-Biotin molecule is cell membrane permeable and can be used to label intracellular proteins; whereas Sulfo-NHS-LC-Biotin is designed to biotinylate extracellular proteins. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin.

Molecular Weight: 454.54 
Spacer Length: 22.4 Å 
CAS Number:72040-63-2 _{(Alternative CAS 1219212-66-4 Biotin-X-NHS)} 
Chemical Formula: C₂₀H₃₀N₄O₆S 

Alternative Names: Biotin-X-NHS; (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester; Biotinamidocaproate N-hydroxysuccinimidyl ester; succinimidyl biotinamido hexanoate; NHS LC Biotin; succinimidyl biotinamidyl hexanoate; succinimidyl biotinamido hexanoate; long chain NHS Biotin 

结构式：

Iodoacetyl-LC-Biotin,93285-75-7,生物素乙酰碘交联剂

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