G418二硫酸盐

分类 其它生化试剂

级别 优级纯GR

产品规格 SIGMA A1720-1G

CAS  108321-42-2

品牌 SIGMA

用途范围 生物科研

保存温度 2-8℃

分子式 C20H40N4O10 · 2H2SO4

分子量 692.71

供应商  上海研伴实业有限公司

G418二硫酸盐        SIGMA A1720-1G

powder, BioReagent, suitable for cell culture

· 产品编号：A1720

· 中文名称：G418 二硫酸盐

· 英文名称：G418 disulfate salt

· CAS号：108321-42-2

· 分子式：C20H40N4O10 · 2H2SO4

· 分子量：692.71

· 保存温度：2-8 °C

· MDL号：MFCD00058314

订货信息：

产品规格价格(RMB)

· A1720-1G    电仪/元

· A1720-5G    电仪/元

· A1720-25G    电仪/元

注：此价格仅供参考，请以SIGMA价格为准，优惠价请电询。

性能描述

Biochem/physiol Actions

G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.

Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.

Mode of Action: Blocks polypeptide synthesis by inhibiting protein synthesis at the level of the 70S and 80S ribosomes. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.

Other Notes

Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.

Application

G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths1. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene2.

G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximay 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.

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