The NEXTFLEX-96^™ RNA-Seq Barcodes are adapters designed for use with the NEXTFLEX^® Rapid RNA-Seq Kit and the NEXTFLEX^® Rapid Directional RNA-Seq Kit. These barcodes are not recommended for use with the NEXTFLEX^® Rapid Directional RNA-seq kit 2.0.

The NEXTFLEX-96^™ RNA-Seq Barcodes provide flexibility and high-throughput capabilities in sequencing applications. They are supplied in 96-well plates. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX-96^™ RNA-Seq Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTFLEX^® index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96^™ RNA-Seq Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different adapters even in the case of an error in the barcode read. These adapters are supplied a 96-well format. Learn more.