ELISA试剂盒血清、血浆样品处理
时间:2012-06-08 阅读:653
ELISA 试剂盒血清、血浆样品处理
Serum (血清标本)- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximay 1000×g. Assay freshly prepared serum immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Plasma (血浆标本)- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.