Cat. Number: Rs-2360R

Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）

 

Background: The GPCR CXCR5 binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain （Wilbanks et al., 2001）. CXCR5 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells （Tabata et al., 2005）. CXCR5 functions as a cofactor with CD4 for HIV entry and Env-mediated cell fusion （Liao et al., 1997）. Binding of CXCL16 to CXCR5 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium （Nanki et al., 2005, Sato et al., 2005）. Millipore’s cloned human CXCR5 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CXCR5 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of CXCR5.

Specificity: · anti-CXCR5 is a rabbit monoclonal antibody unconjugated

· specific for CXCR5 of hu, mo, rat, cow, pig

· epitope mapping near the C terminus of CXCR5 of hu, mo, rat, cow,

pig origin

· use for western blotting, elisa, immunoprecipitation

and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 44kDa

 

Application: · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

 

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.