为了获得更理想的ELISA实验结果，请您

1、在ELISA实验过程中，请配制标准品及工作液A和B，以避免由于不准确稀释而造成的浓度误差；

2、在ELISA实验过程中，酶标板在温育时应加膜覆盖以避免液体蒸发；

3、在ELISA实验过程中，检测液A和检测液B，以及底物溶液在使用前，应置于37℃温育30分钟待用；

4、在ELISA实验过程中，洗板后应尽快进行下一步操作，避免酶标板长时间处于干燥状态。

In order to obtain the optimal ELISA results

1．During ELISA assay operation, Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction and avoid foaming and mix gently until the crystals have compley dissolved;

2．During ELISA assay operation, Proper adhesion of plate sealers during incubation steps is necessary;

3．During ELISA assay operation, Substrate, Detection Reagent A and Detection Reagent B should be incubate for 30 min at 37℃ before use;

4．During ELISA assay operation, After the every step of aspiration / wash, please do not delay entering into the next step in order not to let the assay plate "too dry".