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Anti-Nebulette,肌动蛋白结合蛋白Z盘状蛋白抗体
面议Anti-TNNI3K,*/*蛋白激酶TNN13K抗体
面议Anti-ATG16L2抗体,自噬体ATG16L2蛋白抗体
面议Anti-AFAP/AFAP-110抗体,微丝相关蛋白1抗体
面议Anti-ACPT抗体,睾丸酸性磷酸酶抗体
面议Anti-ANGPTL3/ANG5抗体,血管生成素样蛋白3抗体
面议Anti-AKR1B10抗体,醛糖还原酶相关蛋白质抗体
面议Anti-ASF1A抗体,细胞衰老相关蛋白ASF1A抗体
面议Anti-ASAM/ACAM抗体,脂肪细胞特异性粘附分子抗体
面议Anti-ASAH2抗体,酰基鞘氨醇脱酰酶2抗体
面议Anti-ACVR1B/ALK4抗体,激活素A受体1B抗体
面议Anti-ANKS3抗体,锚蛋白重复及SAM结构域蛋白3抗体
面议博研生物抗体,可应用于IHC、WB、 IF、IP、ELISA等实验,咨询,质量保证,无效免费退换,另外本公司生产经营各种标记二抗、免疫印迹常用试剂、免疫组化常用试剂,咨询!
产品名称:磷酸化ATR抗体,ATR/ACTR(Ser428)抗体
产品编号:BYK-3030R
产品规格: 0.1ml/0.2ml
抗体来源:Rabbit OR Mouse
产品用途:科研实验。
性 状: Lyophilized or Liquid
浓 度: 1mg/1ml
亚 型: IgG
贮 存: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications..
相关标记: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647
更多产品请登入www.boyanbio.com 查询,索取我司相关产品信息内容及说明书。
磷酸化ATR抗体,ATR/ACTR(Ser428)抗体进行免疫组化石蜡切片实验时我司*的步骤:石蜡包埋组织切片3~4μm 厚度
1.烤片: 将待做切片置于切片架上,于60℃恒温烤箱中至少烤1 hr;
2.脱蜡: 切片放入盛有二甲苯的容器中脱蜡3次(即二甲苯Ⅰ、Ⅱ、Ⅲ),每次10 min;
3.水化: 切片经下行酒精水化,无水乙醇5min,95%乙醇2次(每次2min),85%乙醇2 min;75%乙醇2min,自来水冲洗,ddH2O洗2×2min;
4.抗原修复: 根据抗体说明书*方法进行抗原修复,常采用高压、微波(温度达到98~100℃)或酶消化修复法,室温自然冷却,自来水冲洗,ddH2O洗2×2min,TBS洗涤(2×2min)(具体修复方法见附1)* 注:有些抗原勿需修复,直接进入第5步封闭。
5.封闭: 滴加试剂B,37℃湿盒孵育30 min;
6.加一抗:滴加用试剂C(选择合理的工作比例),37℃湿盒孵育2 hr 或4℃过夜;
7.洗涤: TBS-T洗涤(3×5 min);
8.封闭: 滴加试剂B,37℃湿盒孵育10 min;
9.加二抗: 滴加用抗体稀释液稀释的*化二抗(试剂D),37℃湿盒中孵育30 min;
10.洗涤: TBS-T洗涤(3×5 min);
11.封闭: 滴加试剂Tween 20,37℃湿盒孵育封闭20 min;
12.加HRP-SA: 滴加用试剂C稀释的试剂E(1:50~200,终浓度5~20 nM),37℃湿盒中孵育30 min;
13.洗涤: TBS-T洗涤(3×5 min),TBS洗涤(2×5 min);
14.显色:应用DAB溶液(试剂F)显色;
15.复染:自来水充分冲洗,复染,脱水,透明;
16.封片: 待组织标本干后,用试剂缓冲甘油封固剂封片;
17.观察成像: 显微镜下观察成像。
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