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| Catalogue Number | CYT-376 | ||||
| Patent Protected Countries | The Sale of recombinant Rat IL-18 by ProSpec is prohibited in the following countries: United States, Japan, United Kingdom, France, Spain, Italy, Germany, Austria, Belgium, Denmark, Portugal, Sweden, Switzerland, Liechtenstein, Australia, Taiwan, Netherlands, Korea and Canada. | ||||
| Synonyms | Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin. | ||||
| Introduction | IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein （IL18BP） can specifically interact with this cytokine, and thus negatively regulate its biological activity. | ||||
| Description | Interleukin-18 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. The IL-18 is purified by proprietary chromatographic techniques. | ||||
| Source | Escherichia Coli. | ||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||||
| Formulation | Lyophilized from a concentrated （1mg/ml） solution in water containing 0.4 x PBS pH=7.2. | ||||
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||
| Stability | Lyophilized Interleukin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | ||||
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||||
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu. | ||||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells （400,000cells/well） co-stimulated with sub optimal dose of ConA （0.75ug/ml） is 12ng/ml corresponding to a specific activity of 84,000U/mg. The medium was RPMI containing 10% FCS and 50µM 2-ME. | ||||
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard. | ||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
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