品牌
代理商厂商性质
上海市所在地
| Catalogue Number | CYT-411 | |
| Synonyms | Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19. | |
| Introduction | IL19 is a cytokine that belongs to the IL10 cytokine subfamily. IL-19 is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 （STAT3）. A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. | |
| Description | Interleukin-19 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17722 Dalton. The IL-19 is purified by proprietary chromatographic techniques. | |
| Source | Escherichia Coli. | |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation | Lyophilized from a concentrated （1mg/ml） solution in water containing no additives. | |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-19 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability | Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met- Leu-Arg-Arg-Cys. | |
| Biological Activity | The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes （HEK2a cells）. Significant STAT3 activation is observed with >100ng/ml corresponding to a Specific Activity of 10,000IU/mg of IL-19 recombinant mouse. | |
| Protein content | Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard. | |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |
| Related Products |
|
