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产品名称:Anti-MEPE抗体,细胞外基质磷酸化抗体
产品编号:BYK-8689R
英文名称:Anti-MEPE
中文名称:细胞外基质磷酸化抗体
英文别名:Matrix extracellular phosphoglycoprotein; MEPE; MEPE_HUMAN; OF45; Osteoblast/osteocyte factor 45.
英文介绍:MEPE is a 525 amino acid extracellular matrix protein. Expressed in osteocytes and brain, MEPE is a regulator of bone metabolism that is thought to mediate mineralization and demineralization within the osteocyte microenvironment. MEPE contains an RGD cell-attachment motif and shares molecular similarities with several dentin-bone extracellular matrix RGD-containing phosphoglycoproteins, including OPN (osteopontin) and DSP (dentin sialophosphoprotein). Via its ability to control bone mineralization, MEPE is associated with various developmental events such as skeletogenesis, bone regeneration and odontogenesis. MEPE is secreted in hypophosphatemic osteomalacia tumors, suggesting a possible role in the pathophysiology of bone-related cancers.
产品规格:0.1ml/0.2ml
注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗体本公司有单抗和多抗,可用于免疫组化(IHC),免疫印迹(WB),酶联免疫(ELISA),IF、IP、流式细胞术等实验(可来电索取说明)
此产品本公司有标记的一抗出售,相关标记有:Alexa Fluor 350 标记、Alexa Fluor 488 标记、Alexa Fluor 555 标记、Alexa Fluor 647 标记、AP标记、APC标记、Biotin标记、Cy3标记、Cy5标记、Cy5.5标记、Cy7标记、FITC标记、Gold标记、HRP标记、PE标记、PE-Cy3标记、PE-CY5标记、PE-CY5.5标记、PE-CY7标记、RBITC标记
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-MEPE抗体,细胞外基质磷酸化抗体免疫组化操作流程
1 脱蜡和水化:脱蜡前应将组织芯片在室温中放置60分钟或60℃恒温箱中烘烤20分钟。 a 组织芯片置于二甲苯中浸泡10分钟,更换二甲苯后在浸泡10分钟
b 无水乙醇中浸泡五分钟
c 95%乙醇中浸泡五分钟
d 75%乙醇中浸泡五分钟
2 抗原修复:用于福尔马林固定的石蜡包埋组织芯片:
A 抗原热修复
a 高压热修复 在沸水中加入EDTA(ph8.0)或0.01m*缓冲溶液(ph6.0).盖上不锈钢锅盖,但不能锁定。将玻片置于金属染色加上,缓慢加压,是玻片在缓冲液中浸泡五分钟,然后将盖子锁定,小阀门将会升起来。10分钟后除去热源,置入凉水中,当小阀门沉下去后打开盖子。此方法适用于较难检测或核抗原的抗原修复。
b 沸热修复 电炉或水浴锅加热0.01*缓冲液(ph6.0)至95℃左右,放入组织芯片加热10-15分钟。
C 微波炉加热 在微波炉里加热0.01*缓冲液(ph6.0)至沸腾后将组织芯片放入,断电,间隔5-10分钟,反复1-2次。适用的抗原:
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法
常用0.1%*和0.4%*液。*使用前预热37℃,消化时间约为5-30分钟。适用于被固定遮蔽的抗原:包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等
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