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Anti-SALL2抗体,锌指转录蛋白SALL2抗体
英文介绍:The region-specific homeotic gene sal (spalt) of Drosophila encodes a zinc finger protein of unusual but characteristic structure. These unique features were used to isolate sal-like genes from humans. Two sal-like transcription units SALL1 and SALL2, located on chromosomes 16q12.1 and 14q11.1-q12.1 respectively, have been isolated and characterized. SALL1 and SALL2 transcripts are expressed in a limited number of adult organs, including the brain. SALL2 is evenly expressed in different brain areas. Transcripts of both genes can be detected in fetal brain neurons. The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal.
此产品本公司有标记的一抗出售,相关标记有:Alexa Fluor 350 标记、Alexa Fluor 488 标记、Alexa Fluor 555 标记、Alexa Fluor 647 标记、AP标记、APC标记、Biotin标记、Cy3标记、Cy5标记、Cy5.5标记、Cy7标记、FITC标记、Gold标记、HRP标记、PE标记、PE-Cy3标记、PE-CY5标记、PE-CY5.5标记、PE-CY7标记、RBITC标记
注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品名称:Anti-SALL2抗体,锌指转录蛋白SALL2抗体
产品编号:BYK-19370R
英文名称:Anti-SALL2
中文名称:锌指转录蛋白SALL2抗体
英文别名:AI225809;
AW559097;
FLJ10414;
FLJ55746;
HSAL2;
KIAA0360;
mKIAA0360;
Msal-2;
p150 Sal2;
p150(Sal2);
Sal like protein 2;
Sal-2;
Sal-like 2 (Drosophila);
Sal-like 2;
Spalt-like protein 2;
Zinc finger protein SALL2;
Zinc finger protein Spalt-2;
ZNF795.
产品规格:0.1ml/0.2ml
此抗体本公司有单抗和多抗,可用于免疫组化(IHC),免疫印迹(WB),酶联免疫(ELISA),IF、IP、流式细胞术等实验(可来电索取说明)
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-SALL2抗体,锌指转录蛋白SALL2抗体Western Blotting 具体实验步骤:
Western,也称Western blot、Western blotting、Western 印迹,是用抗体检测蛋白的重要方法之一。
Western可以参考如下步骤进行操作:
收集蛋白样品(Protein sample preparation)
可以使用适当的裂解液裂解贴壁细胞、悬浮细胞或组织样品。对于某些特定的亚细胞组份蛋白,例如细胞核蛋白、细胞浆蛋白、线粒体蛋白等,可以参考相关文献提取这些亚细胞组份蛋白,也可以使用试剂盒进行抽提。
收集完蛋白样品后,为确保每个蛋白样品的上样量*,需要测定每个蛋白样品的蛋白浓度。根据所使用的裂解液的不同,需要采用适当的蛋白浓度测定方法。因为不同的蛋白浓度测定方法对于一些去垢剂和还原剂等的兼容性差别很大。
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