Alkaline Phosphatase Assay Kit 

碱性磷酸酶活性检测试剂盒（显色法）

【温馨提示I】：见碱性磷酸酶产品专题，了解更多相关产品资料；

【温馨提示II】：见碱性磷酸酶染色试剂盒产品；

【温馨提示III】：见碱性磷酸酶常用生化底物及试剂盒产品；

 产品关键词：

Phosphatase, Alkaline碱性磷酸酶；ALP/AKP；对硝基苯酚磷酸盐（pNPP）；偶氮偶联法；显色法（定量）；染色法（定性）； 

基本介绍：

? 碱性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一种含两个相同亚基的二聚体，也是一种约含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每个ALP分子含4个锌离子和4个二硫键。镁离子存在情况下，ALP可达到zui高酶活。碱性条件下，ALP能催化磷酸单酯水解，比如对硝基苯酚磷酸盐（pNPP）、磷酸苯酯、葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸、腺苷-5-磷酸等。

? 常见的碱性磷酸酶ALP包括肠道碱性磷酸酶（如小牛肠来源，calf intestinal）、胎盘碱性磷酸酶（placental alkaline phosphatase）、非组织特异性碱性磷酸酶（tissue-nonspecific isozyme, alkaline phosphatase）等。

? ALP常用来偶联抗体和其他蛋白，从而满足ELISA、WB和组织化学检测上的应用。通常用来蛋白的去磷酸化实验，比如酪蛋白和核酸等。当需要高检测灵敏度时也能用来蛋白标记。还可用在DNA或RNA 的5′-末端去磷酸以防止自连。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
 

研究意义：
碱性磷酸酶（ALP）活性的变化与一系列生理和病理事件紧密关联，比如骨骼发育、骨骼相关疾病、ren娠相关疾病和炎症性肠病（inflammatory bowel diseases）等。正因如此，ALP被用作衡量生理特征或疾病的参照指标。另外，ALP活性检测在环境监测、食品质量控制等方面也呈现重要作用。? 干细胞如诱导多潜能干细胞iPS，ALP活性很高，则ALP常被用作iPS诱导成功的标志；? 分化的结肠癌细胞内ALP活性显著提高，则ALP常被当做结肠癌细胞分化程度的指标（定性/定量）；? 血清中ALP水平提高，被称作高碱性磷酸酶血症，此症状被认为和恶性胆管阻塞、原发性胆管硬化、原发性硬化胆管炎、肝淋巴瘤和肝肉瘤等肝胆疾病密切相关；? ALP是成骨细胞的副产物，血清中ALP活性升高和骨头发育密切关联；? 血清ALP活性过低也与一些疾病相关；? 儿童和孕妇血清内ALP活性较常人高一些；血清中ALP活性范围在20-140U/L。

检测原理（碱性磷酸酶活性）：

对硝基苯酚磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。产物黄色越深，代表ALP活性越高，反之则酶活性越低。则通过比色分析可定量检测ALP水平。反应式如下：



检测对象：细胞或组织裂解液或匀浆液、血清、血浆、尿液等样品内源性碱性磷酸酶活性。
 

订购信息：Anaspec国*，>几十篇文献引用，高口碑产品。

试剂盒组分：

   AS-72146 SensoLyte pNPP Alkaline Phosphatase Assay Kit.pdf
 

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引用文献（Anaspec，AS-72146）：

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 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

Anaspec SensoLyte pNPP ALP Assay Kit 碱性磷酸酶活性检测

Anaspec SensoLyte pNPP ALP Assay Kit 碱性磷酸酶活性检测