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人Ⅰ类主要组织兼容性复合体C检测试剂盒定量检测,具有灵敏性高,特异性强,重复性好,性价比高,线性好;操作简单,精确度高;售前售后完善,随时来实验室现场观看,常见ELISA试剂盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文简称:Human Major Histocompatibility Complex Class I A (MHCA) ELISA Kit
人Ⅰ类主要组织兼容性复合体C检测试剂盒注意事项以下四点:
1,封闭:以下包以后是无关的蛋白质溶液浓度高,涂层技术。随函附上使许多不相关的蛋白质充填这些空隙,和搅扰物质的免疫排挤和吸附进程。ELISA试剂盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明胶牛血清;脱脂奶粉,报价相对低廉,人Ⅰ类主要组织兼容性复合体C检测试剂盒可用于高浓度(5%~10%);和一些稀有的运用各种动物血清(首要是为了消除类似的蛋白和酪蛋白等搅扰)。但究竟挑选啥,依据试验的详细实习。
2,洗板:能够说,在中操作,清洗是在首要的关键技术。因为聚苯乙烯等塑料对蛋白质的吸附是普遍存在的,为了完成自在酶与客观相的符号的别离,在板料孔中残留游离和吸附的非特异性搅扰物质的去掉,洗刷,并应将搅扰物质洗刷下来的非特异性吸附。所以在洗衣板会有必定的误差,非常人的要素(当然也有洗衣机除条件),是不完整的或弦清孔,体系的影响是如此灵敏,但不小。
3,人Ⅰ类主要组织兼容性复合体C检测试剂盒加抗体(抗标本和两个):留意的枪头,枪头。样品用稀释稀释,也能够用稀释的闭解。假如要增加两个电阻,但也要留意两个反作业浓度的废物,太高,太低的光的色彩。
4,色彩:色彩体系有许多,咱们开端做,挑选恰当的色彩体系。ELISA试剂盒留意色彩体系酶底物物加硫柳泵节省,组合物加*。7,每次都尽可能的阴和阳空三操控,如剖析疑问的呈现。YB-Pg-Ge 孕酮(Pg) ELISA Kit General Progesterone (Pg) ELISA Kit General 1.56-100ng/mL 0.45ng/mL competitive inhibition 96T/48T 860/602 12 months
YB-PGH2-Ge 前列腺素H2(PGH2) ELISA Kit General Prostaglandin H2 (PGH2) ELISA Kit General 15.625-1000pg/mL 4.2pg/mL competitive inhibition 96T/48T 860/602 12 months
YB-PGI2-Ge 前列环素(PGI2) ELISA Kit General Prostacyclin (PGI2) ELISA Kit General 78.125-5000pg/mL 33.5pg/mL competitive inhibition 96T/48T 860/602 12 months
YB-PGK1-Hu 人磷酸甘油酸酯激酶1(PGK1) ELISA Kit Human Phosphoglycerate Kinase 1 (PGK1) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.123ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PGLYRP1-Hu 人肽聚糖识别蛋白1(PGLYRP1) ELISA Kit Human Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.115ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PGLYRP1-Mu 小鼠肽聚糖识别蛋白1(PGLYRP1) ELISA Kit Mouse Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.9pg/mL sandwich 96T/48T 995/696.5 12 months
YB-PGLYRP2-Hu 人肽聚糖识别蛋白2(PGLYRP2) ELISA Kit Human Peptidoglycan Recognition Protein 2 (PGLYRP2) ELISA Kit Homo sapiens human 1.56-100ng/mL 0.62ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-Pgp-Hu 人渗透性糖蛋白(Pgp) ELISA Kit Human Permeability Glycoprotein (Pgp) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.114ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-Pgp-Mu 小鼠渗透性糖蛋白(Pgp) ELISA Kit Mouse Permeability Glycoprotein (Pgp) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.051ng/mL sandwich 96T/48T 950/665 12 months
YB-PGR-Mu 小鼠孕酮受体(PGR) ELISA Kit Mouse Progesterone Receptor (PGR) ELISA Kit Mus musculus mouse 0.312-20ng/mL 0.128ng/mL competitive inhibition 96T/48T 950/665 12 months
YB-PGR-Hu 人孕酮受体(PGR) ELISA Kit Human Progesterone Receptor (PGR) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.114ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PHB-Hu 人阻抑素(PHB) ELISA Kit Human Prohibitin (PHB) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.122ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PHB-Mu 小鼠阻抑素(PHB) ELISA Kit Mouse Prohibitin (PHB) ELISA Kit Mus musculus mouse 0.312-20ng/mL 0.097ng/mL sandwich 96T/48T 905/633.5 12 months
YB-PHB-Ra 大鼠阻抑素(PHB) ELISA Kit