*抗原
相关标记  HRP   Biotin   Gold   RBITC   AP   FITC   Cy3   Cy5   Cy5.5   Cy7   PE   PE-Cy3   PE-CY5   PE-CY5.5 
 PE-CY7   APC   Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647      
别    名  1 （4 amino 3 5 dichloro phenyl） 2 （tert butylamino）ethanol...
浓    度  20mg/1ml
说 明 书  1ml/1mg 
 
抗体来源  Rabbit 
克隆类型  polyclonal
交叉反应  Clenbuterol Hydrochloride
研究领域  免疫学 药物及化合物 
性    状  Lyophilized or Liquid
免 疫 原  Clenbuterol conjugated to KLH
亚    型  IgG
纯化方法  affinity purified by Protein A
储 存 液  Preservative: 0.01M PBS, pH 7.4
产品应用   ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200
（石蜡切片需做抗原修复）

 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature
for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent
of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
相关产品  免疫组化常用试剂    免疫印迹常用试剂    各种标记二抗
生 产 商  M.c 
产品介绍 Clenbuterol belongs to the group of agonists. In livestock production clenbuterol improves the meat/fat ratio in fattened animals or accelerate the growth. Up to now agonists have not been authorized as adjuvants for fattening. In addition to its lipolytic and anabolic effect, clenbuterol has a relaxing effect on non-striated musculature on which is based its therapeutic use as an antiasthmatic and a tocolytic agent. When employed as a fattening adjuvant, as compared with the therapeutic use, clenbuterol is administered in a 5 to 10 times higher dose. Therefore, it is possible that clenbuterol residues may lead to a risk for consumers after illegal administration.Using the clenbuterol monocalantibody, it is possible to detect clenbuterol and other agonists in urine, muscle and liver both rapidly and with accuracy. Clenbuterol is a long acting beta 2 adrenergic agonist. Like other beta 2 agonists, clenbuterol is believed to act by stimulating production of cyclic AMP through the activation of adenyl cyclase. By definition, Beta 2 agonists have more smooth muscle relaxation activity （bronchial, vascular and uterine smooth muscle） versus its cardiac effects （Beta 1）.
*（俗称瘦肉精，盐酸双氯醇胺，氨哮素，克喘素，氨双氯喘）为一种人工合成的β肾上腺素兴奋剂，具有扩张支气管的作用，常用来防治哮喘、肺气肿等肺部疾病。当其应用剂量达到治疗量的5~10倍时，具有能量重分配作用，可使肌肉合成增加，脂肪沉积减少，因此俗称为“瘦肉精”。在畜牧业生产中一些非法使用者将其作为一种生长促进剂添加到动物饲料中，用以改善胴体的品质，同时也造成食品中*（瘦肉精）的残留。当人们食用*残留的产品后会出现一系列的中毒症状。

利用重氮法将盐酸克伦特罗（CL）分别与载体蛋白钥孔血蓝蛋白（KLH）和牛血清白蛋白（BSA）偶联,以CL-KLH为免疫原免疫BALB/c小鼠,以CL-BSA为检测原,采用杂交瘤技术获得1株针对CL的单克隆抗体细胞株（5D7）。抗体的亚类为IgG1,通过竞争阻断试验证明其具有良好的特异性和敏感性,利用Protein-G Sepharose亲和层析系统纯化单克隆抗体5D7,单克隆抗体5D7对CL的IC50约为4.6 ng/mL