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| Catalogue Number | ENZ-280 | |
| Synonyms | ALT1, Glutamic-pyruvic transaminase 1, GPT 1, Glutamic-alanine transaminase 1, AAT1, ALT, ALAT, SGPT. | |
| Introduction | Alanine transaminase or ALT is a transaminaseenzyme. ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamate It is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase （SGPT） or alanine aminotransferase （ALAT）. Diagnostically, it is almost always measured in units/litre （U/L）. | |
| Description | Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT. The ALT is purified by proprietary chromatographic techniques. | |
| Source | Escherichia Coli. | |
| Physical Appearance | Sterile liquid formulation. | |
| Formulation | The protein was dialyzed against 40mM sodium acetate buffer （pH 5.5）, 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate. | |
| Stability | AAT1 although stable at 10°C for 5 days, should be stored below -18°C. Please prevent freeze-thaw cycles. | |
| Purity | Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | |
| Biological Activity | The specific activity was found to be 1,020 U/mg. | |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |
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