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Human ALKBH2,重组人ALK 烷基化修复同系物2
面议
Human ALDOC,重组人果糖二磷酸醛缩酶A
面议
Human ALDOA,重组人醛缩酶A
面议
Human ALDH5A1,重组人醛脱氢酶3A1
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Human ALDH3A1,重组人醛脱氢酶3A1
面议
Human ALDH2,重组人醛脱氢酶2
面议
Human ALDH1A1,重组人醛脱氢酶1A1
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Human ALAT,重组人*转氨酶
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Human AKR7A3,重组人醛酮还原酶7-A3
面议
Human AKR7A2,重组人醛酮还原酶7-A2
面议
Human AKR1D1,重组人醛酮还原酶1-D1
面议
Human AKR1C4,重组人醛酮还原酶1-C4
面议| Catalogue Number | CYT-305 |
| Synonyms | ATF1, ATF2, HFB1-GDNF, GDNF. |
| Introduction | GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET （rearranged during transfection） protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
| Description | Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton. GDNF is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl. |
| Solubility | It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys. |
| Biological Activity | The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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