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Human ALKBH2,重组人ALK 烷基化修复同系物2
面议Human ALDOC,重组人果糖二磷酸醛缩酶A
面议Human ALDOA,重组人醛缩酶A
面议Human ALDH5A1,重组人醛脱氢酶3A1
面议Human ALDH3A1,重组人醛脱氢酶3A1
面议Human ALDH2,重组人醛脱氢酶2
面议Human ALDH1A1,重组人醛脱氢酶1A1
面议Human ALAT,重组人*转氨酶
面议Human AKR7A3,重组人醛酮还原酶7-A3
面议Human AKR7A2,重组人醛酮还原酶7-A2
面议Human AKR1D1,重组人醛酮还原酶1-D1
面议Human AKR1C4,重组人醛酮还原酶1-C4
面议Catalogue Number | CYT-442 | ||||
Synonyms | Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14. | ||||
Introduction | GDF-5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. | ||||
Description | Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 26.8 kDa. GDF5 is purified by proprietary chromatographic techniques. | ||||
Source | Escherichia Coli. | ||||
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | ||||
Formulation | The protein was lyophilized without any additives. | ||||
Solubility | It is recommended to reconstitute the lyophilized Growth Differentiation Factor-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||
Stability | Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||||
Purity | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | ||||
Biological Activity | GDF-5 activity was determined by the induction of alkaline phosphatase activity in ATDC5 cells: EC50 = 4ug/ml, corresponding to a specific activity of 250units/mg. | ||||
Amino Acid Sequence | APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR. | ||||
Protein content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GDF5 as a Reference Standard. | ||||
References | Title:Growth/differentiation Factor-5 Induces Osteogenic Differentiation of Human Ligamentum Flavum Cells through Activation of ERK1/2 and p38 MAPK. Publication:Cell Physiol Biochem 2010;26:179-186 (DOI: 10.1159/000320526) Link:http://content.karger.com/produktedb/produkte.asp?doi=320526 | ||||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
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