重组磷脂酶A2 p00630蜂毒蛋白|PA2-BVP

100ug重组磷脂酶A2 p00630蜂毒蛋白|PA2-BVP

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2016-06-06 23:36:32
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重组磷脂酶A2 p00630蜂毒蛋白
Recombinant Phospholipase A2 P00630 Bee Venom Protein
PA2-BVP
Catalogue Number ALR-001

详细介绍

Synonyms Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.
Introduction Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.
Description The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids.
Method Purified by proprietary chromatographic technique.
Purity Protein is >90% pure as determined by 10% SDS-PAGE.
Formulation 20mM Tris-HCl, pH 7.2, 1.5M urea and 50% glycerol.
Storage Phospholipase A2 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Applications :
Use as an antigen in ELISA and Western Blots.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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