重组大豆p34蛋白|Soy Bean P34

100ug重组大豆p34蛋白|Soy Bean P34

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2016-05-28 20:35:12
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重组大豆p34蛋白
Recombinant Soy Bean P34 Protein
Soy Bean P34
Catalogue Number ALR-002

详细介绍

Introduction The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.
Description The E.Coli derived recombinant protein contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids, having an MW of 39kDA. The protein is fused to a GST tag at N-terminus.
Method Purified by proprietary chromatographic technique.
Purity Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Formulation 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
Storage Soybean P34 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Applications Use as an antigen in ELISA and Western Blots.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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