Biochips

Cell Adhesion & Cell Culture under Flow, Transmigration, Droplet Generation and Microfluidics Studies

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Cell-Ligａnd / Cell-Protein adhesion 
Under shear flow
Disposable biochip
Cell adhesion, rolling assays on ligand/protein coated channels
Ideal for platelet aggregation & thrombosis assays.

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Cell culture &cell-cell adhesion
Under shear flow
Disposable biochip
Cell culture of endothelial cell monolayers in microchannel flow chambers
Cell-cell adhesion shear flow assays

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Cell-Ligａnd &cell-cell adhesion
Under shear flow
Disposable biochip
Flexibility with glass coverslip supplied unattached.
Glass coverslips may be treated with chelated Cu2+ ion tethered to the high density PEG-coating
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Branching Y-junction at input port
Mimic branching microvascular networks
Chemotaxis assays, dual flow, multilaminar flow & diffusion

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VenaDelta Y2 Branching Y-junction
at input & exit ports
Mimic branching microvascular networks
Chemotaxis assays, dual flow, multilaminar flow & diffusion
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Chemotaxis, Transmigration, Invasion Assays
Under shear flow
Cell adhesion and transmigration under shear flow
Transmigration through microporous membrane into underlying microwell which may contain chemoattractant
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Droplet Generation
Under shear flow
3 Droplet Generators & 1 splitter
High-throughput generation of microdroplets and monodispersed oils/emulsions

HOW DOES IT WORK?

DropChips are fabricated from plastic (acrylic) and have a footprint of 40mm x 50mm (W x L).

DropChips are available with channels pre-treated as hydrophobic or hydrophilic:

• Hydrophobic to achieve water-in-oil droplets.

• Hydrophilic to achieve oil-in-water droplets.

 

DropChips are available in packs of 2, 5 or 10.

Hydrophobic channels:

Water-in-oil droplet generation

Hydrophilic channels:

Oil-in-water droplet generation

As illustrated in the schematic below, each DropChip contains:

• 1 x Flow Splitter and

• 3 x Droplet Generator junctions:

  • 2 x Flow-Focusing junctions, single-injection dispersed phase

  • 1 x Flow Focusing junction with dual-injection dispersed phase

This gives the user flexibility for experiments in flow focusing with single and dual-injection of dispersed phase.

Examples are illustrated in the images below.

DropChip Schematic

3 Flow Focusing Droplet Generator junctions & 1 Flow Splitter

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Droplet Frequency

Droplet Size

DOWNLOADS

DropChip Tech Note

Droplet Generation Kit Brochure

Droplet Frequency

The creation of uniform, mono-dispersed  stable droplets is achieved with microfluidic syringe pumps such as Cellix's ExiGo pump or microfluidic pressure pumps such as Cellix's UniGo or 4U pumps.  Cellix's team conducted 3D scattering measurements to confirm frequency of droplets which can be achieved at defined flow rates as illustrated in the images and frequency graph below.  If you have a specific frequency you want to achieve, contact us for more help.

Droplet Frequency in DropChip  with channel dimensions 70um x 70um

Oil phase: 5uL/min
Water phase: 0.5uL/min

Oil phase: 5uL/min
Water phase: 1uL/min

Oil phase: 5uL/min
Water phase: 2uL/min

Oil phase: 5uL/min
Water phase: 3uL/min

Out of gallery

Droplet Size

There are a variety of different droplet sizes possible and one of the biggest influencing factors of droplet size is the geometry and size of the channel dimensions.  Cellix's team conducted 3D scattering measurements to confirm size and volume of droplets which can be achieved with the two different types of droplet generator contained in the DropChip.  Schematics for each of the droplet generators available on Cellix's Dropchip are illustrated below and the following table provides a summary of the droplet sizes (diameter and volume) which may be achieved depending on the DropChip dimensions.

Droplet Generator junction 1 & 3 (DG1 and DG3):

Flow Focusing, single injection of dispersed phase

Droplet Generator junction 2 (DG2):

Flow Focusing, dual injection of dispersed phase

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APPLICATIONS

Droplet Generation for a wide variety of applications:

• Cell / Protein encapsulation and sorting on-chip, see example below.

• DNA sequencing for target selection / enrichment.

• Sample / reagent mixing and control of reactions on-chip

• DNA amplification

• Biomolecule synthesis

• Diagnostic chips

• Drug discovery

For more details on experimental set-ups, visit our Droplet Generation page.

APPLICATION VIDEOS

Cell (or particle) double encapsulation:

The image below illustrates droplets containing cells, enclosed within a shell of oil.  The following is an example of the experimental set-up to achieve double encapsulation.

Experimental Set-up

• 3 x ExiGo pumps

• 3 x Flow Sensors

• 1 x DropChip (50um x 50um) coated with DropCoat (reagent ensuring hydrophobicity)

• 1 x DropChip (80um x 80um) uncoated, hydrophilic walls

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